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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14701 | 5' | -50.6 | NC_003529.1 | + | 53597 | 0.66 | 0.994753 |
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Target: 5'- ---aCAAUGGUgugaUGCGAGACGUuuuauguuccauGUUGCc -3' miRNA: 3'- caggGUUACCG----ACGCUUUGCA------------CAACG- -5' |
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| 14701 | 5' | -50.6 | NC_003529.1 | + | 46898 | 0.68 | 0.97547 |
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Target: 5'- -aUUCAAUGGacCUGCGAAACaauaGUGUUGUu -3' miRNA: 3'- caGGGUUACC--GACGCUUUG----CACAACG- -5' |
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| 14701 | 5' | -50.6 | NC_003529.1 | + | 96137 | 0.69 | 0.959547 |
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Target: 5'- cGUCCaccggguaGGUGucgauaaaaacGCUGCGAAugGUGUUGa -3' miRNA: 3'- -CAGGg-------UUAC-----------CGACGCUUugCACAACg -5' |
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| 14701 | 5' | -50.6 | NC_003529.1 | + | 137631 | 0.7 | 0.94253 |
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Target: 5'- cGUCgugCAAggacuGCUGCGAAACGUGUcGCg -3' miRNA: 3'- -CAGg--GUUac---CGACGCUUUGCACAaCG- -5' |
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| 14701 | 5' | -50.6 | NC_003529.1 | + | 44279 | 1.14 | 0.004575 |
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Target: 5'- cGUCCCAAUGGCUGCGAAACGUGUUGCg -3' miRNA: 3'- -CAGGGUUACCGACGCUUUGCACAACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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