Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14703 | 5' | -50 | NC_003529.1 | + | 139746 | 0.7 | 0.97365 |
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Target: 5'- aCGGUgA-CCGUUGAGguGGAgcCCACCg -3' miRNA: 3'- gGCUAgUaGGCGACUCguUUU--GGUGG- -5' |
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| 14703 | 5' | -50 | NC_003529.1 | + | 132235 | 0.71 | 0.957179 |
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Target: 5'- aCGAUCAUgCCGgccCUGAGC---ACCAUCa -3' miRNA: 3'- gGCUAGUA-GGC---GACUCGuuuUGGUGG- -5' |
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| 14703 | 5' | -50 | NC_003529.1 | + | 26853 | 0.71 | 0.944485 |
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Target: 5'- gCCGAUUGUCCGUgugaGAGCGAcuCUGCa -3' miRNA: 3'- -GGCUAGUAGGCGa---CUCGUUuuGGUGg -5' |
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| 14703 | 5' | -50 | NC_003529.1 | + | 102310 | 0.72 | 0.912337 |
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Target: 5'- uUCGAUUcuGUCCGaguUGAGCAu--CCGCCa -3' miRNA: 3'- -GGCUAG--UAGGCg--ACUCGUuuuGGUGG- -5' |
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| 14703 | 5' | -50 | NC_003529.1 | + | 128018 | 0.75 | 0.821956 |
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Target: 5'- -gGAUCGUCCGC---GCGAAACUGCCu -3' miRNA: 3'- ggCUAGUAGGCGacuCGUUUUGGUGG- -5' |
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| 14703 | 5' | -50 | NC_003529.1 | + | 139848 | 0.77 | 0.705779 |
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Target: 5'- gCCGAaagCAUagaGUUGAGCGAAGCCGCUa -3' miRNA: 3'- -GGCUa--GUAgg-CGACUCGUUUUGGUGG- -5' |
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| 14703 | 5' | -50 | NC_003529.1 | + | 58261 | 1.07 | 0.017427 |
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Target: 5'- aCCGAUC-UCCGCUGAGCAAAACCACCg -3' miRNA: 3'- -GGCUAGuAGGCGACUCGUUUUGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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