miRNA: 3'- auUGGGCGGCuUGGGCGGcaACGgua -5'
miRNA: 3'- auuGGGCGGCUUG------GGCGGCAacGGUa -5'
miRNA: 3'- aUUGGGC-----GGCUUGgGCGGCAa-CGGUa -5'
miRNA: 3'- aUUGGGCGGcUUGGG---CGGCAACGgua -5'
miRNA: 3'- -AUUGGGCGGCUUGGGCGGCAACGGUA- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14704 | 5' | -59.3 | NC_003529.1 | + | 5722 | 0.66 | 0.745489 |
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Target: 5'- -uGCCCGCCGcACCCGUC--UGCa-- -3' miRNA: 3'- auUGGGCGGCuUGGGCGGcaACGgua -5' |
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| 14704 | 5' | -59.3 | NC_003529.1 | + | 127635 | 0.68 | 0.6056 |
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Target: 5'- --cCCCGCCGAACagcgagCCGCCGU--CCAg -3' miRNA: 3'- auuGGGCGGCUUG------GGCGGCAacGGUa -5' |
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| 14704 | 5' | -59.3 | NC_003529.1 | + | 101570 | 0.7 | 0.516172 |
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Target: 5'- cGGCCCGacacaUCGAACgCGCCGUcgGCCAc -3' miRNA: 3'- aUUGGGC-----GGCUUGgGCGGCAa-CGGUa -5' |
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| 14704 | 5' | -59.3 | NC_003529.1 | + | 22705 | 0.7 | 0.50653 |
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Target: 5'- aAGCCCGCC-AACCCucaGCUGUUGCa-- -3' miRNA: 3'- aUUGGGCGGcUUGGG---CGGCAACGgua -5' |
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| 14704 | 5' | -59.3 | NC_003529.1 | + | 65462 | 1.03 | 0.003095 |
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Target: 5'- gUAACCCGCCGAACCCGCCGUUGCCAUu -3' miRNA: 3'- -AUUGGGCGGCUUGGGCGGCAACGGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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