Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14705 | 3' | -52.5 | NC_003529.1 | + | 131581 | 0.68 | 0.953018 |
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Target: 5'- --aUGUGCGCCAA-GUGauaGACAAGa -3' miRNA: 3'- aagACACGCGGUUgCACag-CUGUUCc -5' |
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| 14705 | 3' | -52.5 | NC_003529.1 | + | 49071 | 0.68 | 0.929441 |
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Target: 5'- aUCUuuUGCGaaGGCGUGUaCGACGGGGa -3' miRNA: 3'- aAGAc-ACGCggUUGCACA-GCUGUUCC- -5' |
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| 14705 | 3' | -52.5 | NC_003529.1 | + | 34592 | 0.7 | 0.871356 |
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Target: 5'- gUUUGUGCGCUggcaacgcaacGAUGUgGUCGGgGAGGg -3' miRNA: 3'- aAGACACGCGG-----------UUGCA-CAGCUgUUCC- -5' |
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| 14705 | 3' | -52.5 | NC_003529.1 | + | 63108 | 0.7 | 0.871356 |
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Target: 5'- ----aUGCGUCAGUGUGUCGACAuGGg -3' miRNA: 3'- aagacACGCGGUUGCACAGCUGUuCC- -5' |
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| 14705 | 3' | -52.5 | NC_003529.1 | + | 109862 | 0.72 | 0.776458 |
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Target: 5'- -aUUGcGCGaCCGACGUGUCGACAu-- -3' miRNA: 3'- aaGACaCGC-GGUUGCACAGCUGUucc -5' |
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| 14705 | 3' | -52.5 | NC_003529.1 | + | 68100 | 0.97 | 0.035996 |
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Target: 5'- uUUC-GUGCGCCAACGUGUCGACAAGGc -3' miRNA: 3'- -AAGaCACGCGGUUGCACAGCUGUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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