Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14706 | 3' | -49.4 | NC_003529.1 | + | 152129 | 0.66 | 0.995665 |
Target: 5'- -aGAUUguGAAGAGUAACagUGCCGCUc -3' miRNA: 3'- ugCUGAauCUUCUCGUUG--AUGGCGAu -5' |
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14706 | 3' | -49.4 | NC_003529.1 | + | 10482 | 0.68 | 0.98256 |
Target: 5'- uCGAUgauGAAGAGCAGCUugCGg-- -3' miRNA: 3'- uGCUGaauCUUCUCGUUGAugGCgau -5' |
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14706 | 3' | -49.4 | NC_003529.1 | + | 72519 | 0.69 | 0.972609 |
Target: 5'- gGCGACaaUGGccGAGCGAagauguCUGCCGCUGu -3' miRNA: 3'- -UGCUGa-AUCuuCUCGUU------GAUGGCGAU- -5' |
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14706 | 3' | -49.4 | NC_003529.1 | + | 76214 | 0.72 | 0.919816 |
Target: 5'- gACGGCgcAGGAGcAGCAGCgggcGCCGCa- -3' miRNA: 3'- -UGCUGaaUCUUC-UCGUUGa---UGGCGau -5' |
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14706 | 3' | -49.4 | NC_003529.1 | + | 72745 | 1.08 | 0.01302 |
Target: 5'- gACGACUUAGAAGAGCAACUACCGCUAa -3' miRNA: 3'- -UGCUGAAUCUUCUCGUUGAUGGCGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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