Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14706 | 5' | -55.1 | NC_003529.1 | + | 3556 | 0.66 | 0.903921 |
Target: 5'- gGCGGCGGCGucGGCAUCguuguuUUCGCU--GCa -3' miRNA: 3'- aUGUCGCCGU--CUGUAG------AAGCGAgcCG- -5' |
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14706 | 5' | -55.1 | NC_003529.1 | + | 86915 | 0.66 | 0.903921 |
Target: 5'- cGCAGCGcCAG-UGUCUUCGUcCGGUu -3' miRNA: 3'- aUGUCGCcGUCuGUAGAAGCGaGCCG- -5' |
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14706 | 5' | -55.1 | NC_003529.1 | + | 123693 | 0.66 | 0.903921 |
Target: 5'- aGCAGCGaugcGCAuuCGUUUUCGCUuugUGGCg -3' miRNA: 3'- aUGUCGC----CGUcuGUAGAAGCGA---GCCG- -5' |
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14706 | 5' | -55.1 | NC_003529.1 | + | 141259 | 0.67 | 0.897452 |
Target: 5'- -uUAGCGGCAGuuCGUCgUCGCUaCuGCu -3' miRNA: 3'- auGUCGCCGUCu-GUAGaAGCGA-GcCG- -5' |
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14706 | 5' | -55.1 | NC_003529.1 | + | 142417 | 0.67 | 0.886608 |
Target: 5'- uUGCGGCGGCAGuggcguguccgaucuGCGUCguuUCGauucgCGGUg -3' miRNA: 3'- -AUGUCGCCGUC---------------UGUAGa--AGCga---GCCG- -5' |
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14706 | 5' | -55.1 | NC_003529.1 | + | 697 | 0.67 | 0.86924 |
Target: 5'- cACGG-GGCAGccACcgCcUCGCUUGGCc -3' miRNA: 3'- aUGUCgCCGUC--UGuaGaAGCGAGCCG- -5' |
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14706 | 5' | -55.1 | NC_003529.1 | + | 43079 | 0.67 | 0.86924 |
Target: 5'- cAUAGCGGCAGuuuCAUCgUCGCagUCGa- -3' miRNA: 3'- aUGUCGCCGUCu--GUAGaAGCG--AGCcg -5' |
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14706 | 5' | -55.1 | NC_003529.1 | + | 25453 | 0.68 | 0.837535 |
Target: 5'- cAUAGUGGCcGGCGgguuugccgcuuUCUU-GCUCGGCu -3' miRNA: 3'- aUGUCGCCGuCUGU------------AGAAgCGAGCCG- -5' |
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14706 | 5' | -55.1 | NC_003529.1 | + | 77670 | 0.68 | 0.837535 |
Target: 5'- cUAUAuGCGGCGGACAa---CGaCUCGGCc -3' miRNA: 3'- -AUGU-CGCCGUCUGUagaaGC-GAGCCG- -5' |
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14706 | 5' | -55.1 | NC_003529.1 | + | 131774 | 0.71 | 0.70604 |
Target: 5'- aGCAGCacgucgucGGCGGGCAcgcguUCUUCGcCUUGGUc -3' miRNA: 3'- aUGUCG--------CCGUCUGU-----AGAAGC-GAGCCG- -5' |
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14706 | 5' | -55.1 | NC_003529.1 | + | 72709 | 1.09 | 0.003177 |
Target: 5'- cUACAGCGGCAGACAUCUUCGCUCGGCc -3' miRNA: 3'- -AUGUCGCCGUCUGUAGAAGCGAGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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