Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14707 | 5' | -55.7 | NC_003529.1 | + | 76269 | 0.66 | 0.891998 |
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Target: 5'- aAGCGGAGGguACAGGcggcguuccaGCGGUuaccaucgucguccCGGCAGg -3' miRNA: 3'- -UUGCUUCCguUGUUC----------CGCCA--------------GCCGUU- -5' |
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| 14707 | 5' | -55.7 | NC_003529.1 | + | 38655 | 0.66 | 0.87263 |
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Target: 5'- cAUGGucauGGCcgUuuGGCGGUCGGCGu -3' miRNA: 3'- uUGCUu---CCGuuGuuCCGCCAGCCGUu -5' |
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| 14707 | 5' | -55.7 | NC_003529.1 | + | 126356 | 0.66 | 0.871129 |
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Target: 5'- aGACGAGgauGGCAACGguuccugAGGCGGUgccgacggaccacUGGCAc -3' miRNA: 3'- -UUGCUU---CCGUUGU-------UCCGCCA-------------GCCGUu -5' |
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| 14707 | 5' | -55.7 | NC_003529.1 | + | 67453 | 0.66 | 0.868098 |
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Target: 5'- gAACGccGGCGcuuaucaccgauugcGCaAAGGCGG-CGGCAAu -3' miRNA: 3'- -UUGCuuCCGU---------------UG-UUCCGCCaGCCGUU- -5' |
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| 14707 | 5' | -55.7 | NC_003529.1 | + | 104109 | 0.67 | 0.815062 |
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Target: 5'- -gUGAAGGCAugAcGGUGG-CGGCu- -3' miRNA: 3'- uuGCUUCCGUugUuCCGCCaGCCGuu -5' |
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| 14707 | 5' | -55.7 | NC_003529.1 | + | 92699 | 0.7 | 0.656773 |
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Target: 5'- uAACGAAGGUggUGGcGGCGGUggUGGCGGu -3' miRNA: 3'- -UUGCUUCCGuuGUU-CCGCCA--GCCGUU- -5' |
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| 14707 | 5' | -55.7 | NC_003529.1 | + | 1389 | 0.73 | 0.492145 |
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Target: 5'- cGGCGGAGGUGGCGGcGGUGG-CGGCGGu -3' miRNA: 3'- -UUGCUUCCGUUGUU-CCGCCaGCCGUU- -5' |
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| 14707 | 5' | -55.7 | NC_003529.1 | + | 86959 | 1.03 | 0.005609 |
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Target: 5'- cAACGAAGGCAACAAGGCGGUCGGCAAc -3' miRNA: 3'- -UUGCUUCCGUUGUUCCGCCAGCCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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