Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14708 | 5' | -48.6 | NC_003529.1 | + | 124066 | 0.66 | 0.999392 |
Target: 5'- gCAAUugUAUCGAcGCACcaaGGgCGa -3' miRNA: 3'- gGUUAugAUAGCUaCGUGaagCCgGC- -5' |
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14708 | 5' | -48.6 | NC_003529.1 | + | 77351 | 0.66 | 0.999392 |
Target: 5'- aCUAAUGCgaagcaGGUGgGCgcugUCGGCCa -3' miRNA: 3'- -GGUUAUGauag--CUACgUGa---AGCCGGc -5' |
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14708 | 5' | -48.6 | NC_003529.1 | + | 132283 | 0.66 | 0.999248 |
Target: 5'- gCCGGUGCcaccgucUCGAaGCucguaggcaACUUCGGCCa -3' miRNA: 3'- -GGUUAUGau-----AGCUaCG---------UGAAGCCGGc -5' |
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14708 | 5' | -48.6 | NC_003529.1 | + | 56441 | 0.66 | 0.999248 |
Target: 5'- gCCGcaACgaucgCGAUGCAg-UCGGCCa -3' miRNA: 3'- -GGUuaUGaua--GCUACGUgaAGCCGGc -5' |
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14708 | 5' | -48.6 | NC_003529.1 | + | 59572 | 0.67 | 0.998631 |
Target: 5'- cCCGcgGCaca-GGUGCAg-UCGGCCGg -3' miRNA: 3'- -GGUuaUGauagCUACGUgaAGCCGGC- -5' |
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14708 | 5' | -48.6 | NC_003529.1 | + | 146304 | 0.67 | 0.998014 |
Target: 5'- -uGAUGCUcaCGAUGUACacgUUGGCCu -3' miRNA: 3'- ggUUAUGAuaGCUACGUGa--AGCCGGc -5' |
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14708 | 5' | -48.6 | NC_003529.1 | + | 152841 | 0.67 | 0.9975 |
Target: 5'- gCCGAcccUACUuuggcuaagauggcGUCGGUGCACauguaCGGCUGg -3' miRNA: 3'- -GGUU---AUGA--------------UAGCUACGUGaa---GCCGGC- -5' |
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14708 | 5' | -48.6 | NC_003529.1 | + | 75623 | 0.68 | 0.99718 |
Target: 5'- aCGGuUugUAUCuagGCGCUUUGGCCa -3' miRNA: 3'- gGUU-AugAUAGcuaCGUGAAGCCGGc -5' |
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14708 | 5' | -48.6 | NC_003529.1 | + | 101573 | 0.68 | 0.996665 |
Target: 5'- cCCGAcAC-AUCGAacGCGCcgUCGGCCa -3' miRNA: 3'- -GGUUaUGaUAGCUa-CGUGa-AGCCGGc -5' |
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14708 | 5' | -48.6 | NC_003529.1 | + | 141830 | 0.69 | 0.990489 |
Target: 5'- gCCAc-AUUGUUG-UGCGCaUCGGCCGg -3' miRNA: 3'- -GGUuaUGAUAGCuACGUGaAGCCGGC- -5' |
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14708 | 5' | -48.6 | NC_003529.1 | + | 111023 | 0.69 | 0.988553 |
Target: 5'- aCAAU-CUAUCGAUGCACgcguucaauaugUGGCgCGg -3' miRNA: 3'- gGUUAuGAUAGCUACGUGaa----------GCCG-GC- -5' |
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14708 | 5' | -48.6 | NC_003529.1 | + | 98323 | 0.7 | 0.987636 |
Target: 5'- cCCAGU---GUCGGUGCACgccaUCGcGCCa -3' miRNA: 3'- -GGUUAugaUAGCUACGUGa---AGC-CGGc -5' |
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14708 | 5' | -48.6 | NC_003529.1 | + | 61398 | 0.7 | 0.982169 |
Target: 5'- gUCAA-ACUA-CGAuaUGCACgugUCGGCCGc -3' miRNA: 3'- -GGUUaUGAUaGCU--ACGUGa--AGCCGGC- -5' |
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14708 | 5' | -48.6 | NC_003529.1 | + | 141231 | 0.73 | 0.936827 |
Target: 5'- gCCAGcGCUAUCGgcGCGaugUUUGGCCGc -3' miRNA: 3'- -GGUUaUGAUAGCuaCGUg--AAGCCGGC- -5' |
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14708 | 5' | -48.6 | NC_003529.1 | + | 58497 | 0.8 | 0.621712 |
Target: 5'- gCGAUAUgaguaacgGAUGCACUUCGGCCGg -3' miRNA: 3'- gGUUAUGauag----CUACGUGAAGCCGGC- -5' |
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14708 | 5' | -48.6 | NC_003529.1 | + | 93978 | 1.13 | 0.009891 |
Target: 5'- cCCAAUACUAUCGAUGCACUUCGGCCGa -3' miRNA: 3'- -GGUUAUGAUAGCUACGUGAAGCCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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