Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14709 | 5' | -46.6 | NC_003529.1 | + | 43146 | 0.66 | 0.999614 |
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Target: 5'- cGCGUUAACAucGUGUucgcgCACGUGa--- -3' miRNA: 3'- aCGCGAUUGUuuCACG-----GUGUACaaaa -5' |
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| 14709 | 5' | -46.6 | NC_003529.1 | + | 18336 | 0.67 | 0.999511 |
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Target: 5'- -cUGUUGACGAAGUGCCAUGUc---- -3' miRNA: 3'- acGCGAUUGUUUCACGGUGUAcaaaa -5' |
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| 14709 | 5' | -46.6 | NC_003529.1 | + | 148441 | 0.68 | 0.997884 |
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Target: 5'- uUGCGU---CAGAGUGCCGUAUGUg-- -3' miRNA: 3'- -ACGCGauuGUUUCACGGUGUACAaaa -5' |
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| 14709 | 5' | -46.6 | NC_003529.1 | + | 37351 | 0.69 | 0.996374 |
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Target: 5'- gGCGgaGGCAAAGUGCUGCuuUGUc-- -3' miRNA: 3'- aCGCgaUUGUUUCACGGUGu-ACAaaa -5' |
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| 14709 | 5' | -46.6 | NC_003529.1 | + | 96576 | 0.69 | 0.994944 |
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Target: 5'- aGCGUguuuCAGAGUGCUGCAaUGUUa- -3' miRNA: 3'- aCGCGauu-GUUUCACGGUGU-ACAAaa -5' |
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| 14709 | 5' | -46.6 | NC_003529.1 | + | 32343 | 0.7 | 0.990318 |
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Target: 5'- uUGCGCUugcguguacacgguGGCGGAucGCCACAUGUUg- -3' miRNA: 3'- -ACGCGA--------------UUGUUUcaCGGUGUACAAaa -5' |
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| 14709 | 5' | -46.6 | NC_003529.1 | + | 95521 | 1.06 | 0.027492 |
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Target: 5'- aUGCGCUAACAAAGUGCCACAUGUUUUu -3' miRNA: 3'- -ACGCGAUUGUUUCACGGUGUACAAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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