miRNA: 3'- aUACCGCaCAGa--CGUGUAcCCUGUGc -5'
miRNA: 3'- -AUACCGCACA---------------GACG-UGUACcCUGUGc -5'
miRNA: 3'- auaCCGCA----CAGACGUg--UACCcUGUGC- -5'
miRNA: 3'- -AUACCGCACAGACGUGUACCCUGUGC- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14710 | 3' | -54.4 | NC_003529.1 | + | 114733 | 0.66 | 0.945636 |
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Target: 5'- aGUGGCGgGUUgacGCACGUcGGAUACu -3' miRNA: 3'- aUACCGCaCAGa--CGUGUAcCCUGUGc -5' |
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| 14710 | 3' | -54.4 | NC_003529.1 | + | 91769 | 0.67 | 0.922364 |
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Target: 5'- aUGUGGCGUGUaaacgguugcuguacUUGCgACAUGuGGCGCa -3' miRNA: 3'- -AUACCGCACA---------------GACG-UGUACcCUGUGc -5' |
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| 14710 | 3' | -54.4 | NC_003529.1 | + | 148600 | 0.67 | 0.901792 |
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Target: 5'- --aGGCGUcaccGUCUGCGagaAUGG-ACACGa -3' miRNA: 3'- auaCCGCA----CAGACGUg--UACCcUGUGC- -5' |
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| 14710 | 3' | -54.4 | NC_003529.1 | + | 126516 | 1.08 | 0.004377 |
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Target: 5'- uUAUGGCGUGUCUGCACAUGGGACACGa -3' miRNA: 3'- -AUACCGCACAGACGUGUACCCUGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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