Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14710 | 5' | -51.9 | NC_003529.1 | + | 12587 | 0.66 | 0.98297 |
Target: 5'- cGGCuguGCGcCGAGUGUauAGCGuCGUCGCc -3' miRNA: 3'- -CUGuuuCGC-GUUCACA--UCGU-GCGGUG- -5' |
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14710 | 5' | -51.9 | NC_003529.1 | + | 14422 | 0.66 | 0.98086 |
Target: 5'- uGCAuuuuGUGCAAGUG-AGCACGUUg- -3' miRNA: 3'- cUGUuu--CGCGUUCACaUCGUGCGGug -5' |
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14710 | 5' | -51.9 | NC_003529.1 | + | 82751 | 0.66 | 0.98086 |
Target: 5'- uGCAGuuuGGCGCGuauauagaAGUGUacgaucugAGCACGCCcGCu -3' miRNA: 3'- cUGUU---UCGCGU--------UCACA--------UCGUGCGG-UG- -5' |
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14710 | 5' | -51.9 | NC_003529.1 | + | 76207 | 0.67 | 0.970442 |
Target: 5'- aGCAAuggacGGCGCAGGaGcAGCagcggGCGCCGCa -3' miRNA: 3'- cUGUU-----UCGCGUUCaCaUCG-----UGCGGUG- -5' |
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14710 | 5' | -51.9 | NC_003529.1 | + | 137358 | 0.67 | 0.960328 |
Target: 5'- uGAUAAGGCcguGCuuuUGUGcGCGCGCCGCu -3' miRNA: 3'- -CUGUUUCG---CGuucACAU-CGUGCGGUG- -5' |
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14710 | 5' | -51.9 | NC_003529.1 | + | 49857 | 0.68 | 0.952389 |
Target: 5'- --gGAAGaaauauuGCGAGUagAGCGCGCCACg -3' miRNA: 3'- cugUUUCg------CGUUCAcaUCGUGCGGUG- -5' |
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14710 | 5' | -51.9 | NC_003529.1 | + | 44275 | 0.68 | 0.943448 |
Target: 5'- -cCAAuGGCuGCGAaacGUGUuGCGCGCCACa -3' miRNA: 3'- cuGUU-UCG-CGUU---CACAuCGUGCGGUG- -5' |
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14710 | 5' | -51.9 | NC_003529.1 | + | 69762 | 0.68 | 0.938593 |
Target: 5'- aACAAcGCGCGAuGUGUGGUauugguGCGCgACa -3' miRNA: 3'- cUGUUuCGCGUU-CACAUCG------UGCGgUG- -5' |
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14710 | 5' | -51.9 | NC_003529.1 | + | 98313 | 0.69 | 0.897321 |
Target: 5'- uGCAAuuGCGCccAGUGUcgguGCACGCCAUc -3' miRNA: 3'- cUGUUu-CGCGu-UCACAu---CGUGCGGUG- -5' |
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14710 | 5' | -51.9 | NC_003529.1 | + | 49626 | 0.7 | 0.868152 |
Target: 5'- aGACAAAGUccuCGAGUGUGGCAUGUuuuuauCGCa -3' miRNA: 3'- -CUGUUUCGc--GUUCACAUCGUGCG------GUG- -5' |
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14710 | 5' | -51.9 | NC_003529.1 | + | 126482 | 1.1 | 0.004624 |
Target: 5'- gGACAAAGCGCAAGUGUAGCACGCCACa -3' miRNA: 3'- -CUGUUUCGCGUUCACAUCGUGCGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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