Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14714 | 3' | -54.1 | NC_003529.1 | + | 111195 | 0.66 | 0.947067 |
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Target: 5'- aGUCGAGUGUGCCGUcaacGAuGUgaugC-CGCAg -3' miRNA: 3'- -CAGCUCGUACGGCG----CUuCAa---GuGCGU- -5' |
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| 14714 | 3' | -54.1 | NC_003529.1 | + | 70695 | 0.66 | 0.942538 |
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Target: 5'- uGUCGAuGCGUGUCGCcucGUacagcuugUCGCGCAu -3' miRNA: 3'- -CAGCU-CGUACGGCGcuuCA--------AGUGCGU- -5' |
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| 14714 | 3' | -54.1 | NC_003529.1 | + | 143338 | 0.66 | 0.937764 |
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Target: 5'- uGUCGGGCAUucgGCCGCGcggauuGGUgUCugGUc -3' miRNA: 3'- -CAGCUCGUA---CGGCGCu-----UCA-AGugCGu -5' |
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| 14714 | 3' | -54.1 | NC_003529.1 | + | 51096 | 0.68 | 0.903931 |
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Target: 5'- uUUGAGCGUGaaGCGAAGUcCA-GCAc -3' miRNA: 3'- cAGCUCGUACggCGCUUCAaGUgCGU- -5' |
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| 14714 | 3' | -54.1 | NC_003529.1 | + | 20767 | 0.68 | 0.890696 |
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Target: 5'- uUCG-GCGUcGCCGacauuGggGUUCGCGCc -3' miRNA: 3'- cAGCuCGUA-CGGCg----CuuCAAGUGCGu -5' |
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| 14714 | 3' | -54.1 | NC_003529.1 | + | 148831 | 0.68 | 0.890696 |
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Target: 5'- -gCGGGCAaguUGCCGCGGA----GCGCAa -3' miRNA: 3'- caGCUCGU---ACGGCGCUUcaagUGCGU- -5' |
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| 14714 | 3' | -54.1 | NC_003529.1 | + | 33624 | 0.69 | 0.845497 |
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Target: 5'- gGUCGuucuAGgGUGCgGUGguGUUCGCGCAa -3' miRNA: 3'- -CAGC----UCgUACGgCGCuuCAAGUGCGU- -5' |
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| 14714 | 3' | -54.1 | NC_003529.1 | + | 137631 | 0.71 | 0.764766 |
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Target: 5'- cGUCGuGCAaggacUGCUGCGAAacgugUCGCGCGa -3' miRNA: 3'- -CAGCuCGU-----ACGGCGCUUca---AGUGCGU- -5' |
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| 14714 | 3' | -54.1 | NC_003529.1 | + | 20712 | 1.09 | 0.003982 |
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Target: 5'- cGUCGAGCAUGCCGCGAAGUUCACGCAg -3' miRNA: 3'- -CAGCUCGUACGGCGCUUCAAGUGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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