Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14717 | 3' | -54.5 | NC_003529.1 | + | 22871 | 0.66 | 0.955051 |
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Target: 5'- gGUUGGcgggcUuuGCGAUCACGUCcUGgGCa -3' miRNA: 3'- -UAACC-----AggCGCUAGUGCGGuAUgCGg -5' |
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| 14717 | 3' | -54.5 | NC_003529.1 | + | 74881 | 0.66 | 0.946749 |
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Target: 5'- --aGGUUCGUuacuuUCACGUCGUugGCg -3' miRNA: 3'- uaaCCAGGCGcu---AGUGCGGUAugCGg -5' |
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| 14717 | 3' | -54.5 | NC_003529.1 | + | 58845 | 0.66 | 0.942243 |
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Target: 5'- --cGGUCgaGCGAUCgACGCCGguuuCGUa -3' miRNA: 3'- uaaCCAGg-CGCUAG-UGCGGUau--GCGg -5' |
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| 14717 | 3' | -54.5 | NC_003529.1 | + | 45455 | 0.67 | 0.916077 |
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Target: 5'- -cUGuGUCCuCGAUCAUGUCGcgcacGCGCCu -3' miRNA: 3'- uaAC-CAGGcGCUAGUGCGGUa----UGCGG- -5' |
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| 14717 | 3' | -54.5 | NC_003529.1 | + | 35596 | 0.67 | 0.910115 |
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Target: 5'- -gUGG-CCGCGuUCG-GCCA-GCGCCu -3' miRNA: 3'- uaACCaGGCGCuAGUgCGGUaUGCGG- -5' |
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| 14717 | 3' | -54.5 | NC_003529.1 | + | 123759 | 0.68 | 0.89747 |
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Target: 5'- -aUGGaUCGCGAUUGCGCUc-GCGCUg -3' miRNA: 3'- uaACCaGGCGCUAGUGCGGuaUGCGG- -5' |
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| 14717 | 3' | -54.5 | NC_003529.1 | + | 21700 | 0.69 | 0.829463 |
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Target: 5'- -gUGGUCCgGCGAUCuugGCGauguggCAUACGUCu -3' miRNA: 3'- uaACCAGG-CGCUAG---UGCg-----GUAUGCGG- -5' |
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| 14717 | 3' | -54.5 | NC_003529.1 | + | 23417 | 0.71 | 0.737006 |
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Target: 5'- --cGGUCCGCGAcCACGCaCAaaugAUGUCc -3' miRNA: 3'- uaaCCAGGCGCUaGUGCG-GUa---UGCGG- -5' |
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| 14717 | 3' | -54.5 | NC_003529.1 | + | 51408 | 0.74 | 0.563294 |
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Target: 5'- --gGGUCCGCGAUCGCuGCUcgaacuGUACuuuGCCa -3' miRNA: 3'- uaaCCAGGCGCUAGUG-CGG------UAUG---CGG- -5' |
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| 14717 | 3' | -54.5 | NC_003529.1 | + | 21779 | 1.11 | 0.003089 |
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Target: 5'- cAUUGGUCCGCGAUCACGCCAUACGCCg -3' miRNA: 3'- -UAACCAGGCGCUAGUGCGGUAUGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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