Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14719 | 3' | -47.7 | NC_003529.1 | + | 49398 | 0.66 | 0.999857 |
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Target: 5'- aCGAAauaagCGuCCGGCGAUGCUGa-- -3' miRNA: 3'- gGUUUgaa--GCuGGCUGUUACGACaca -5' |
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| 14719 | 3' | -47.7 | NC_003529.1 | + | 37704 | 0.67 | 0.999402 |
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Target: 5'- aCAGAUcaUUCGACCagauuGCAA-GCUGUGUu -3' miRNA: 3'- gGUUUG--AAGCUGGc----UGUUaCGACACA- -5' |
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| 14719 | 3' | -47.7 | NC_003529.1 | + | 93978 | 0.67 | 0.999402 |
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Target: 5'- cCCAAuacuaucgaugcACUUCGGCCGACAGcGCcGa-- -3' miRNA: 3'- -GGUU------------UGAAGCUGGCUGUUaCGaCaca -5' |
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| 14719 | 3' | -47.7 | NC_003529.1 | + | 3896 | 0.67 | 0.998883 |
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Target: 5'- gCGAugUUUGaugcuGCCGGCGAUGUUgGUGUu -3' miRNA: 3'- gGUUugAAGC-----UGGCUGUUACGA-CACA- -5' |
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| 14719 | 3' | -47.7 | NC_003529.1 | + | 141051 | 0.68 | 0.99718 |
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Target: 5'- gCCAAACaUCGcGCCGAUAGcGCUGg-- -3' miRNA: 3'- -GGUUUGaAGC-UGGCUGUUaCGACaca -5' |
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| 14719 | 3' | -47.7 | NC_003529.1 | + | 58139 | 1.11 | 0.016094 |
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Target: 5'- aCCAAACUUCGACCGACAAUGCUGUGUu -3' miRNA: 3'- -GGUUUGAAGCUGGCUGUUACGACACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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