Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14720 | 5' | -50.7 | NC_003529.1 | + | 66404 | 0.66 | 0.994753 |
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Target: 5'- gGACGuGGUCGUCAAacccGCCGAUc-- -3' miRNA: 3'- aCUGC-CUAGCAGUUaca-CGGCUGuua -5' |
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| 14720 | 5' | -50.7 | NC_003529.1 | + | 131494 | 0.67 | 0.989347 |
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Target: 5'- cGAC-GAUCGUCAccagaGCCGACAGc -3' miRNA: 3'- aCUGcCUAGCAGUuaca-CGGCUGUUa -5' |
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| 14720 | 5' | -50.7 | NC_003529.1 | + | 23396 | 0.67 | 0.987871 |
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Target: 5'- -uAUGGAUCGUauuGAUGUcgagggGCCGACAGa -3' miRNA: 3'- acUGCCUAGCAg--UUACA------CGGCUGUUa -5' |
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| 14720 | 5' | -50.7 | NC_003529.1 | + | 144526 | 0.67 | 0.987871 |
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Target: 5'- gUGAUGGA--GUCAAaGUauGCCGACAAa -3' miRNA: 3'- -ACUGCCUagCAGUUaCA--CGGCUGUUa -5' |
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| 14720 | 5' | -50.7 | NC_003529.1 | + | 72129 | 0.7 | 0.94716 |
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Target: 5'- cGAUcgGGAUCGUCGGUGUaGUCGuGCAAc -3' miRNA: 3'- aCUG--CCUAGCAGUUACA-CGGC-UGUUa -5' |
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| 14720 | 5' | -50.7 | NC_003529.1 | + | 94463 | 0.7 | 0.937643 |
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Target: 5'- cGAUGGAUUGUCcc---GCCGACGAa -3' miRNA: 3'- aCUGCCUAGCAGuuacaCGGCUGUUa -5' |
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| 14720 | 5' | -50.7 | NC_003529.1 | + | 101750 | 0.71 | 0.90933 |
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Target: 5'- cGACGGcgCGuUCGAUGUGUCGGg--- -3' miRNA: 3'- aCUGCCuaGC-AGUUACACGGCUguua -5' |
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| 14720 | 5' | -50.7 | NC_003529.1 | + | 63121 | 0.73 | 0.84266 |
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Target: 5'- -uACGGAaacuaucaugCGUCAGUGUGUCGACAu- -3' miRNA: 3'- acUGCCUa---------GCAGUUACACGGCUGUua -5' |
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| 14720 | 5' | -50.7 | NC_003529.1 | + | 131697 | 0.73 | 0.84266 |
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Target: 5'- aUGGCGGcacaAUCGUCGGUG-GCCG-CGAUg -3' miRNA: 3'- -ACUGCC----UAGCAGUUACaCGGCuGUUA- -5' |
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| 14720 | 5' | -50.7 | NC_003529.1 | + | 59099 | 1.06 | 0.0141 |
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Target: 5'- gUGACGGAUCGUCAAUGUGCCGACAAUu -3' miRNA: 3'- -ACUGCCUAGCAGUUACACGGCUGUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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