miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
14723 5' -54 NC_003529.1 + 114734 0.66 0.94433
Target:  5'- -gUGGCg--G-GUUGACGCACGUCGg -3'
miRNA:   3'- guGCCGagaCgCAACUGCGUGUAGUa -5'
14723 5' -54 NC_003529.1 + 68923 0.68 0.892904
Target:  5'- cCACGGUgccgaaUGCGUUGGCGCcggaAUCGg -3'
miRNA:   3'- -GUGCCGag----ACGCAACUGCGug--UAGUa -5'
14723 5' -54 NC_003529.1 + 38807 0.7 0.785947
Target:  5'- cCACGGCaaUGUGUUGAuCGCGCAauuguUCAa -3'
miRNA:   3'- -GUGCCGagACGCAACU-GCGUGU-----AGUa -5'
14723 5' -54 NC_003529.1 + 32504 0.7 0.776458
Target:  5'- aGCGGCUUUGCGU----GCACGUCGc -3'
miRNA:   3'- gUGCCGAGACGCAacugCGUGUAGUa -5'
14723 5' -54 NC_003529.1 + 51125 0.74 0.591916
Target:  5'- aCACGGacaauuaUGUGUUGGCGCGCAUCu- -3'
miRNA:   3'- -GUGCCgag----ACGCAACUGCGUGUAGua -5'
14723 5' -54 NC_003529.1 + 93873 1.06 0.006053
Target:  5'- gCACGGCUCUGCGUUGACGCACAUCAUg -3'
miRNA:   3'- -GUGCCGAGACGCAACUGCGUGUAGUA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.