Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14725 | 3' | -52.2 | NC_003529.1 | + | 29945 | 0.66 | 0.984955 |
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Target: 5'- gAC-CGcUGUGGCGacaagaGgUGCACUUGCa -3' miRNA: 3'- -UGuGCaACACCGCa-----CgAUGUGAACGc -5' |
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| 14725 | 3' | -52.2 | NC_003529.1 | + | 60734 | 0.66 | 0.98306 |
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Target: 5'- uACAUGUcuuaUGUGGCGU-CUAaucaguGCUUGCa -3' miRNA: 3'- -UGUGCA----ACACCGCAcGAUg-----UGAACGc -5' |
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| 14725 | 3' | -52.2 | NC_003529.1 | + | 105391 | 0.66 | 0.980991 |
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Target: 5'- --uCGUUGgaUGGCG-GCUGCAUcauUUGCGc -3' miRNA: 3'- uguGCAAC--ACCGCaCGAUGUG---AACGC- -5' |
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| 14725 | 3' | -52.2 | NC_003529.1 | + | 91778 | 0.66 | 0.976295 |
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Target: 5'- uCAUGaugcaUGUGGCGUguaaacgguuGCUGUACUUGCGa -3' miRNA: 3'- uGUGCa----ACACCGCA----------CGAUGUGAACGC- -5' |
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| 14725 | 3' | -52.2 | NC_003529.1 | + | 13011 | 0.67 | 0.970799 |
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Target: 5'- cGCuuGUUGUGGUG-GCUgcgGCGCUUGa- -3' miRNA: 3'- -UGugCAACACCGCaCGA---UGUGAACgc -5' |
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| 14725 | 3' | -52.2 | NC_003529.1 | + | 11867 | 0.67 | 0.970799 |
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Target: 5'- -gACGUuugggUGUGGCgGUGCUGCAgacuauguUUUGUGg -3' miRNA: 3'- ugUGCA-----ACACCG-CACGAUGU--------GAACGC- -5' |
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| 14725 | 3' | -52.2 | NC_003529.1 | + | 98503 | 0.69 | 0.912337 |
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Target: 5'- gGCGCGa--UGGCGUGCaccgACACUggGCGc -3' miRNA: 3'- -UGUGCaacACCGCACGa---UGUGAa-CGC- -5' |
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| 14725 | 3' | -52.2 | NC_003529.1 | + | 857 | 0.72 | 0.803986 |
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Target: 5'- --uCGUUgGUGGUGUGCUGCgACUUGUu -3' miRNA: 3'- uguGCAA-CACCGCACGAUG-UGAACGc -5' |
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| 14725 | 3' | -52.2 | NC_003529.1 | + | 126294 | 1.08 | 0.007001 |
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Target: 5'- gACACGUUGUGGCGUGCUACACUUGCGc -3' miRNA: 3'- -UGUGCAACACCGCACGAUGUGAACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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