Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1475 | 5' | -52.5 | NC_001335.1 | + | 43914 | 0.66 | 0.832217 |
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Target: 5'- -uGCUCAGuGUCGCUgucgACGggGcGCUGa -3' miRNA: 3'- acUGAGUC-CAGCGA----UGCaaCuCGACg -5' |
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| 1475 | 5' | -52.5 | NC_001335.1 | + | 9348 | 0.67 | 0.773371 |
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Target: 5'- ----aCAGGUCGCUGCGUacacGGGaUUGCc -3' miRNA: 3'- acugaGUCCAGCGAUGCAa---CUC-GACG- -5' |
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| 1475 | 5' | -52.5 | NC_001335.1 | + | 39057 | 0.69 | 0.653051 |
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Target: 5'- gGAUcCGGGUCucccaGCU-CGUaGAGCUGCg -3' miRNA: 3'- aCUGaGUCCAG-----CGAuGCAaCUCGACG- -5' |
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| 1475 | 5' | -52.5 | NC_001335.1 | + | 14287 | 0.69 | 0.641754 |
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Target: 5'- uUGACUgCGgcGGUCgGCUACGUguacgucgGuGCUGCa -3' miRNA: 3'- -ACUGA-GU--CCAG-CGAUGCAa-------CuCGACG- -5' |
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| 1475 | 5' | -52.5 | NC_001335.1 | + | 7287 | 0.72 | 0.476567 |
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Target: 5'- gGGCUCgAGGUCGCUgccucaauACGgccaccGGCUGCg -3' miRNA: 3'- aCUGAG-UCCAGCGA--------UGCaac---UCGACG- -5' |
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| 1475 | 5' | -52.5 | NC_001335.1 | + | 17165 | 0.76 | 0.310828 |
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Target: 5'- cGGC-CAGGUCGCagGCGcgGuGCUGCa -3' miRNA: 3'- aCUGaGUCCAGCGa-UGCaaCuCGACG- -5' |
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| 1475 | 5' | -52.5 | NC_001335.1 | + | 17010 | 0.93 | 0.023853 |
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Target: 5'- cUGACUCAGGUCGCUACGUUGAuCgGCa -3' miRNA: 3'- -ACUGAGUCCAGCGAUGCAACUcGaCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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