Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1478 | 5' | -59.3 | NC_001335.1 | + | 50052 | 0.66 | 0.502476 |
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Target: 5'- cUGGG--AUGGAgacuucuucagccCCACCuauguggccgcuuGGGGUCGAGUc -3' miRNA: 3'- -ACCCacUACCU-------------GGUGG-------------CCCCAGCUCG- -5' |
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| 1478 | 5' | -59.3 | NC_001335.1 | + | 30126 | 0.66 | 0.501467 |
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Target: 5'- cUGGcGaagcUGGUGGACCcccggcccuucgagGCCGGcGGguUCGGGCa -3' miRNA: 3'- -ACC-C----ACUACCUGG--------------UGGCC-CC--AGCUCG- -5' |
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| 1478 | 5' | -59.3 | NC_001335.1 | + | 33655 | 0.67 | 0.45516 |
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Target: 5'- cGGGaGAUGG-CCGCUGaGGUCGAc- -3' miRNA: 3'- aCCCaCUACCuGGUGGCcCCAGCUcg -5' |
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| 1478 | 5' | -59.3 | NC_001335.1 | + | 33901 | 0.69 | 0.348551 |
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Target: 5'- aUGGGuUGGUGcaGACCACCc-GGUUGGGCu -3' miRNA: 3'- -ACCC-ACUAC--CUGGUGGccCCAGCUCG- -5' |
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| 1478 | 5' | -59.3 | NC_001335.1 | + | 1368 | 0.69 | 0.340509 |
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Target: 5'- -cGGUGG-GGGgCACCGGGGUguugGGGCu -3' miRNA: 3'- acCCACUaCCUgGUGGCCCCAg---CUCG- -5' |
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| 1478 | 5' | -59.3 | NC_001335.1 | + | 16472 | 0.7 | 0.300879 |
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Target: 5'- gGGGUGAUcgcggucagcaucGGGCCgagcugcuggaacACCGGGGU-GAGUc -3' miRNA: 3'- aCCCACUA-------------CCUGG-------------UGGCCCCAgCUCG- -5' |
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| 1478 | 5' | -59.3 | NC_001335.1 | + | 19626 | 0.71 | 0.248265 |
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Target: 5'- cGGGaUGAUGGACCACUaccGGGUCa--- -3' miRNA: 3'- aCCC-ACUACCUGGUGGc--CCCAGcucg -5' |
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| 1478 | 5' | -59.3 | NC_001335.1 | + | 19551 | 1.12 | 0.000268 |
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Target: 5'- gUGGGUGAUGGACCACCGGGGUCGAGCa -3' miRNA: 3'- -ACCCACUACCUGGUGGCCCCAGCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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