Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1479 | 3' | -57 | NC_001335.1 | + | 30038 | 0.66 | 0.553162 |
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Target: 5'- -cGGC-CUCGAaGGGCCGGGGg---- -3' miRNA: 3'- uuCCGcGAGCUaCCUGGCCCUacuac -5' |
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| 1479 | 3' | -57 | NC_001335.1 | + | 39786 | 0.66 | 0.552082 |
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Target: 5'- -cGGCGCUgaCGAcaucgcagaccgcUGGGCCuggGGGAUGAa- -3' miRNA: 3'- uuCCGCGA--GCU-------------ACCUGG---CCCUACUac -5' |
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| 1479 | 3' | -57 | NC_001335.1 | + | 1372 | 0.67 | 0.531704 |
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Target: 5'- ----aGCUCGGUGGggggcACCGGGGUGuUGg -3' miRNA: 3'- uuccgCGAGCUACC-----UGGCCCUACuAC- -5' |
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| 1479 | 3' | -57 | NC_001335.1 | + | 21607 | 0.67 | 0.521087 |
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Target: 5'- -cGGCuaUCGcUGGGCCGGGG-GAUc -3' miRNA: 3'- uuCCGcgAGCuACCUGGCCCUaCUAc -5' |
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| 1479 | 3' | -57 | NC_001335.1 | + | 1137 | 0.67 | 0.521087 |
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Target: 5'- aGAGGCGCUgGAgcccccGGAUCGGGcgGc-- -3' miRNA: 3'- -UUCCGCGAgCUa-----CCUGGCCCuaCuac -5' |
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| 1479 | 3' | -57 | NC_001335.1 | + | 7463 | 0.67 | 0.500111 |
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Target: 5'- cAGGCGaCUCuGUcGAcaCCGGGAUGAUGu -3' miRNA: 3'- uUCCGC-GAGcUAcCU--GGCCCUACUAC- -5' |
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| 1479 | 3' | -57 | NC_001335.1 | + | 25038 | 0.77 | 0.117895 |
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Target: 5'- -uGGUGCgugcgUGGUGGGCCGGGAUGGg- -3' miRNA: 3'- uuCCGCGa----GCUACCUGGCCCUACUac -5' |
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| 1479 | 3' | -57 | NC_001335.1 | + | 19609 | 1.07 | 0.000837 |
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Target: 5'- cAAGGCGCUCGAUGGACCGGGAUGAUGg -3' miRNA: 3'- -UUCCGCGAGCUACCUGGCCCUACUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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