Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1481 | 3' | -52.8 | NC_001335.1 | + | 17218 | 0.66 | 0.827736 |
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Target: 5'- cGAGGGCCGUC-GCcaGCGCcggGAUGUGc -3' miRNA: 3'- -CUUCUGGUAGuCGagUGCGa--CUGCAC- -5' |
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| 1481 | 3' | -52.8 | NC_001335.1 | + | 17365 | 0.66 | 0.812531 |
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Target: 5'- uGGGugucuCCAUCGGCggcgcgcucaucggCGCGCUGGCGa- -3' miRNA: 3'- cUUCu----GGUAGUCGa-------------GUGCGACUGCac -5' |
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| 1481 | 3' | -52.8 | NC_001335.1 | + | 46879 | 0.67 | 0.757702 |
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Target: 5'- --uGGCCAUCAGCUCgaACGCUuuCGc- -3' miRNA: 3'- cuuCUGGUAGUCGAG--UGCGAcuGCac -5' |
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| 1481 | 3' | -52.8 | NC_001335.1 | + | 22904 | 0.68 | 0.703358 |
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Target: 5'- --cGACCGUCGGCaUCAC-CgacGGCGUGu -3' miRNA: 3'- cuuCUGGUAGUCG-AGUGcGa--CUGCAC- -5' |
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| 1481 | 3' | -52.8 | NC_001335.1 | + | 31925 | 0.68 | 0.681017 |
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Target: 5'- uGGAGuCCGUCAGCUCugagauugACGCUGucuACGa- -3' miRNA: 3'- -CUUCuGGUAGUCGAG--------UGCGAC---UGCac -5' |
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| 1481 | 3' | -52.8 | NC_001335.1 | + | 21892 | 0.68 | 0.669767 |
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Target: 5'- aGAGGCCAUCGGC----GCUGACGg- -3' miRNA: 3'- cUUCUGGUAGUCGagugCGACUGCac -5' |
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| 1481 | 3' | -52.8 | NC_001335.1 | + | 1776 | 0.73 | 0.420467 |
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Target: 5'- --uGACCAUCGGCgacuCGCUGAUGUc -3' miRNA: 3'- cuuCUGGUAGUCGagu-GCGACUGCAc -5' |
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| 1481 | 3' | -52.8 | NC_001335.1 | + | 3190 | 0.77 | 0.248494 |
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Target: 5'- gGggGACCAUCAGCUCcCGCcgcucgccuUGGCGa- -3' miRNA: 3'- -CuuCUGGUAGUCGAGuGCG---------ACUGCac -5' |
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| 1481 | 3' | -52.8 | NC_001335.1 | + | 8949 | 0.87 | 0.054294 |
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Target: 5'- uGGAGACCAUCGGCUgguucCGCGCUGACGg- -3' miRNA: 3'- -CUUCUGGUAGUCGA-----GUGCGACUGCac -5' |
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| 1481 | 3' | -52.8 | NC_001335.1 | + | 20021 | 1.08 | 0.001665 |
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Target: 5'- cGAAGACCAUCAGCUCACGCUGACGUGu -3' miRNA: 3'- -CUUCUGGUAGUCGAGUGCGACUGCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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