Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1483 | 5' | -56.3 | NC_001335.1 | + | 17120 | 0.66 | 0.651878 |
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Target: 5'- -gGUGACCagucugGCCCCGCaCAuCCCggcGCUGg -3' miRNA: 3'- cgCACUGGa-----UGGGGUG-GUuGGG---UGAU- -5' |
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| 1483 | 5' | -56.3 | NC_001335.1 | + | 25937 | 0.66 | 0.607767 |
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Target: 5'- uGCuugGGCC-ACCCCguuggccgcaGCCAGCUCGCUGa -3' miRNA: 3'- -CGca-CUGGaUGGGG----------UGGUUGGGUGAU- -5' |
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| 1483 | 5' | -56.3 | NC_001335.1 | + | 41500 | 0.67 | 0.553161 |
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Target: 5'- aGCG-GAUC--CCCCGCCGACCCuCg- -3' miRNA: 3'- -CGCaCUGGauGGGGUGGUUGGGuGau -5' |
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| 1483 | 5' | -56.3 | NC_001335.1 | + | 31771 | 0.68 | 0.531703 |
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Target: 5'- gGCGUGGCggUACgUCAUCAagGCCCGCUGg -3' miRNA: 3'- -CGCACUGg-AUGgGGUGGU--UGGGUGAU- -5' |
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| 1483 | 5' | -56.3 | NC_001335.1 | + | 18892 | 0.68 | 0.50011 |
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Target: 5'- aGgGUGGgCUaaACCCCACCGACCagUACa- -3' miRNA: 3'- -CgCACUgGA--UGGGGUGGUUGG--GUGau -5' |
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| 1483 | 5' | -56.3 | NC_001335.1 | + | 2368 | 0.68 | 0.489763 |
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Target: 5'- cGCGgcgaaguucaGCCUcgACCUCGCUGGCCCGCUGg -3' miRNA: 3'- -CGCac--------UGGA--UGGGGUGGUUGGGUGAU- -5' |
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| 1483 | 5' | -56.3 | NC_001335.1 | + | 3206 | 0.69 | 0.449431 |
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Target: 5'- --aUGGCCgACCUCGgCAACCCACUc -3' miRNA: 3'- cgcACUGGaUGGGGUgGUUGGGUGAu -5' |
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| 1483 | 5' | -56.3 | NC_001335.1 | + | 45017 | 0.7 | 0.392564 |
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Target: 5'- ---cGGCCUGCCCCACUcgguagcaAACCUugUGa -3' miRNA: 3'- cgcaCUGGAUGGGGUGG--------UUGGGugAU- -5' |
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| 1483 | 5' | -56.3 | NC_001335.1 | + | 40296 | 0.71 | 0.348055 |
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Target: 5'- --aUGACCacuccaaacgcuaUGCCCCgcaagACCAACCCACUGc -3' miRNA: 3'- cgcACUGG-------------AUGGGG-----UGGUUGGGUGAU- -5' |
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| 1483 | 5' | -56.3 | NC_001335.1 | + | 21866 | 1.09 | 0.000743 |
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Target: 5'- uGCGUGACCUACCCCACCAACCCACUAg -3' miRNA: 3'- -CGCACUGGAUGGGGUGGUUGGGUGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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