miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
14832 5' -51.3 NC_003607.1 + 3198 0.67 0.196643
Target:  5'- cGAGGGCGUaG-CGUGgGCUAuCGCUu -3'
miRNA:   3'- uCUUCCGUAaCaGCGCgUGAUuGCGG- -5'
14832 5' -51.3 NC_003607.1 + 1592 0.68 0.177922
Target:  5'- aAGAAGGUu---UCGCGUcguacuGCUGGCGCg -3'
miRNA:   3'- -UCUUCCGuaacAGCGCG------UGAUUGCGg -5'
14832 5' -51.3 NC_003607.1 + 1386 0.68 0.166337
Target:  5'- uGggGGCAagcaacUGUUGCGCGguuuCUGucCGCCa -3'
miRNA:   3'- uCuuCCGUa-----ACAGCGCGU----GAUu-GCGG- -5'
14832 5' -51.3 NC_003607.1 + 931 0.7 0.118029
Target:  5'- -cGGGGCgAUUGUCGaCGCcCU-ACGCCu -3'
miRNA:   3'- ucUUCCG-UAACAGC-GCGuGAuUGCGG- -5'
14832 5' -51.3 NC_003607.1 + 1058 0.72 0.080158
Target:  5'- aAGggGGCAUcguccgcgaucUGUUGCGCugUGGugguuucggucUGCCu -3'
miRNA:   3'- -UCuuCCGUA-----------ACAGCGCGugAUU-----------GCGG- -5'
14832 5' -51.3 NC_003607.1 + 3928 1.12 0.000044
Target:  5'- gAGAAGGCAUUGUCGCGCACUAACGCCc -3'
miRNA:   3'- -UCUUCCGUAACAGCGCGUGAUUGCGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.