Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1484 | 3' | -56.8 | NC_001335.1 | + | 28944 | 0.66 | 0.609079 |
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Target: 5'- cGCGGGaCGUgaccugUCGGGCUCUGaUUCGCc -3' miRNA: 3'- uUGUCCgGUA------GGCCCGAGAC-AAGUGc -5' |
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| 1484 | 3' | -56.8 | NC_001335.1 | + | 2941 | 0.66 | 0.576054 |
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Target: 5'- -uCGGGCCAUCUGccagucggucGGCUCguaGUacuUCACGg -3' miRNA: 3'- uuGUCCGGUAGGC----------CCGAGa--CA---AGUGC- -5' |
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| 1484 | 3' | -56.8 | NC_001335.1 | + | 27082 | 0.66 | 0.576054 |
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Target: 5'- cAACuGGCCA-CCGGGCaUCUGgaugaaCACc -3' miRNA: 3'- -UUGuCCGGUaGGCCCG-AGACaa----GUGc -5' |
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| 1484 | 3' | -56.8 | NC_001335.1 | + | 26229 | 0.67 | 0.532708 |
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Target: 5'- -cCAGGCCccgaggCgCGGGUUCUGgcCGCGa -3' miRNA: 3'- uuGUCCGGua----G-GCCCGAGACaaGUGC- -5' |
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| 1484 | 3' | -56.8 | NC_001335.1 | + | 22216 | 0.67 | 0.500979 |
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Target: 5'- cAGCGGGCCGUCuUGGaGCgc-GUUCAUGa -3' miRNA: 3'- -UUGUCCGGUAG-GCC-CGagaCAAGUGC- -5' |
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| 1484 | 3' | -56.8 | NC_001335.1 | + | 21961 | 1.07 | 0.000795 |
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Target: 5'- gAACAGGCCAUCCGGGCUCUGUUCACGa -3' miRNA: 3'- -UUGUCCGGUAGGCCCGAGACAAGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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