Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1485 | 3' | -57.9 | NC_001335.1 | + | 48979 | 0.66 | 0.545042 |
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Target: 5'- --aGUCuUCGcCGAGAUCUCCCgAGACa -3' miRNA: 3'- cucCAGuAGCcGCUCUAGGGGG-UCUG- -5' |
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| 1485 | 3' | -57.9 | NC_001335.1 | + | 15422 | 0.67 | 0.5032 |
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Target: 5'- ---aUCAgcUCGGCGAGGgcgcggcuUCCCCCAGuuGCg -3' miRNA: 3'- cuccAGU--AGCCGCUCU--------AGGGGGUC--UG- -5' |
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| 1485 | 3' | -57.9 | NC_001335.1 | + | 44486 | 0.67 | 0.5032 |
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Target: 5'- -uGGcCggCGGCGGGuucagguacGUCCCCCgAGGCa -3' miRNA: 3'- cuCCaGuaGCCGCUC---------UAGGGGG-UCUG- -5' |
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| 1485 | 3' | -57.9 | NC_001335.1 | + | 30969 | 0.67 | 0.486852 |
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Target: 5'- aGGGuUCAcgccguaguucUCGGCGAGAUCCuucugcuucauaccgCCCAGGu -3' miRNA: 3'- cUCC-AGU-----------AGCCGCUCUAGG---------------GGGUCUg -5' |
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| 1485 | 3' | -57.9 | NC_001335.1 | + | 2752 | 0.67 | 0.462816 |
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Target: 5'- -cGGUC--UGGUGAcGAUCCCCgAGAUg -3' miRNA: 3'- cuCCAGuaGCCGCU-CUAGGGGgUCUG- -5' |
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| 1485 | 3' | -57.9 | NC_001335.1 | + | 41573 | 0.68 | 0.452986 |
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Target: 5'- cGAGG--GUCGGCGGGGgauccgcuaCCCCCGGuCu -3' miRNA: 3'- -CUCCagUAGCCGCUCUa--------GGGGGUCuG- -5' |
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| 1485 | 3' | -57.9 | NC_001335.1 | + | 9526 | 0.72 | 0.256487 |
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Target: 5'- aAGGg---CGGCGAcguUCCCCCGGACa -3' miRNA: 3'- cUCCaguaGCCGCUcu-AGGGGGUCUG- -5' |
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| 1485 | 3' | -57.9 | NC_001335.1 | + | 30924 | 0.8 | 0.073081 |
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Target: 5'- cGGGUCAUUaGUGGuGAUCCCCCGGACg -3' miRNA: 3'- cUCCAGUAGcCGCU-CUAGGGGGUCUG- -5' |
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| 1485 | 3' | -57.9 | NC_001335.1 | + | 22221 | 1.1 | 0.000426 |
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Target: 5'- cGAGGUCAUCGGCGAGAUCCCCCAGACg -3' miRNA: 3'- -CUCCAGUAGCCGCUCUAGGGGGUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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