Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1486 | 3' | -56.7 | NC_001335.1 | + | 21269 | 0.69 | 0.425082 |
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Target: 5'- aGUGCGGCUuUCGGCAcgucgacuggcUCCGGGA-GCUg -3' miRNA: 3'- -UACGUCGAuGGCCGU-----------AGGUCCUgUGG- -5' |
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| 1486 | 3' | -56.7 | NC_001335.1 | + | 1184 | 0.69 | 0.406145 |
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Target: 5'- uUGCGuGCU-CCGGU-UCCGGGACcgggGCCg -3' miRNA: 3'- uACGU-CGAuGGCCGuAGGUCCUG----UGG- -5' |
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| 1486 | 3' | -56.7 | NC_001335.1 | + | 16557 | 0.69 | 0.387747 |
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Target: 5'- uGUGCucGCUACCGGCAcgcaggcguUCCu-GACGCUg -3' miRNA: 3'- -UACGu-CGAUGGCCGU---------AGGucCUGUGG- -5' |
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| 1486 | 3' | -56.7 | NC_001335.1 | + | 51830 | 0.71 | 0.327812 |
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Target: 5'- uAUGCgcggcacgGGCUGCCGGgacuGUCCAGcGugACCa -3' miRNA: 3'- -UACG--------UCGAUGGCCg---UAGGUC-CugUGG- -5' |
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| 1486 | 3' | -56.7 | NC_001335.1 | + | 36693 | 0.71 | 0.288639 |
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Target: 5'- gGUGCAGgUagucgacugGCCGGCGgucgugguugcccUCguGGACACCa -3' miRNA: 3'- -UACGUCgA---------UGGCCGU-------------AGguCCUGUGG- -5' |
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| 1486 | 3' | -56.7 | NC_001335.1 | + | 36985 | 0.72 | 0.268053 |
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Target: 5'- -gGCGGUcAUCGGCuacAUCgGGGACACCc -3' miRNA: 3'- uaCGUCGaUGGCCG---UAGgUCCUGUGG- -5' |
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| 1486 | 3' | -56.7 | NC_001335.1 | + | 38162 | 0.77 | 0.121996 |
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Target: 5'- -cGCAGCUACCG---UCCAGuGACGCCa -3' miRNA: 3'- uaCGUCGAUGGCcguAGGUC-CUGUGG- -5' |
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| 1486 | 3' | -56.7 | NC_001335.1 | + | 12554 | 0.79 | 0.094411 |
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Target: 5'- uUGguGUUGCgCGGUuUCCGGGGCACCu -3' miRNA: 3'- uACguCGAUG-GCCGuAGGUCCUGUGG- -5' |
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| 1486 | 3' | -56.7 | NC_001335.1 | + | 22411 | 1.09 | 0.000521 |
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Target: 5'- aAUGCAGCUACCGGCAUCCAGGACACCu -3' miRNA: 3'- -UACGUCGAUGGCCGUAGGUCCUGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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