Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14908 | 5' | -51.9 | NC_003634.1 | + | 822 | 0.66 | 0.277909 |
Target: 5'- cUCCAUCCCcCAccCAUCUC-UCAcCCUa -3' miRNA: 3'- -AGGUAGGGuGUc-GUAGAGuAGUuGGA- -5' |
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14908 | 5' | -51.9 | NC_003634.1 | + | 2672 | 0.67 | 0.229599 |
Target: 5'- cUCUAUCCUGCAGCAUaC-CAUCccgacacAGCCa -3' miRNA: 3'- -AGGUAGGGUGUCGUA-GaGUAG-------UUGGa -5' |
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14908 | 5' | -51.9 | NC_003634.1 | + | 2747 | 0.67 | 0.223103 |
Target: 5'- cCCAUCCCAaaaaacaAGUgccuUCUCA-CAGCCUu -3' miRNA: 3'- aGGUAGGGUg------UCGu---AGAGUaGUUGGA- -5' |
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14908 | 5' | -51.9 | NC_003634.1 | + | 5221 | 1.08 | 0.000109 |
Target: 5'- cUCCAUCCCACAGCAUCUCAUCAACCUc -3' miRNA: 3'- -AGGUAGGGUGUCGUAGAGUAGUUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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