Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1491 | 5' | -48.2 | NC_001335.1 | + | 23906 | 0.66 | 0.976804 |
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Target: 5'- --cCUgGAGGaAACACCAcGgcGUCCGc -3' miRNA: 3'- cuuGAgUUCC-UUGUGGUaCaaCAGGC- -5' |
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| 1491 | 5' | -48.2 | NC_001335.1 | + | 8073 | 0.66 | 0.972065 |
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Target: 5'- aAGCUCAAGGucAACGCCAgcccgaacaacccggUGgcGUUCGa -3' miRNA: 3'- cUUGAGUUCC--UUGUGGU---------------ACaaCAGGC- -5' |
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| 1491 | 5' | -48.2 | NC_001335.1 | + | 41999 | 0.67 | 0.963706 |
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Target: 5'- aGAugUCAccuAGGAuccAgACCGUGUcGUCCu -3' miRNA: 3'- -CUugAGU---UCCU---UgUGGUACAaCAGGc -5' |
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| 1491 | 5' | -48.2 | NC_001335.1 | + | 6329 | 0.67 | 0.946155 |
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Target: 5'- --cCUCGAGGAuGC-CCAUcgUGUCCGa -3' miRNA: 3'- cuuGAGUUCCU-UGuGGUAcaACAGGC- -5' |
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| 1491 | 5' | -48.2 | NC_001335.1 | + | 8948 | 0.68 | 0.941023 |
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Target: 5'- cGACUCGgauGGccuugGACACCcgGUUGaUCCGg -3' miRNA: 3'- cUUGAGUu--CC-----UUGUGGuaCAAC-AGGC- -5' |
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| 1491 | 5' | -48.2 | NC_001335.1 | + | 1373 | 0.68 | 0.929836 |
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Target: 5'- cAGCUCGgugGGGGGCACCgggGUGUUGgggCUGc -3' miRNA: 3'- cUUGAGU---UCCUUGUGG---UACAACa--GGC- -5' |
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| 1491 | 5' | -48.2 | NC_001335.1 | + | 14500 | 0.7 | 0.83657 |
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Target: 5'- cGAACUCAGGGAGCguGCCucggcugGUGUgg-CCGa -3' miRNA: 3'- -CUUGAGUUCCUUG--UGG-------UACAacaGGC- -5' |
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| 1491 | 5' | -48.2 | NC_001335.1 | + | 28055 | 1.11 | 0.0039 |
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Target: 5'- gGAACUCAAGGAACACCAUGUUGUCCGg -3' miRNA: 3'- -CUUGAGUUCCUUGUGGUACAACAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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