Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14927 | 3' | -40.9 | NC_003663.2 | + | 131207 | 0.7 | 1 |
Target: 5'- ---aUCgaAAAGAACGGAGAAUuguuGUCUGu -3' miRNA: 3'- acaaAGa-UUUCUUGCCUCUUA----UAGAU- -5' |
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14927 | 3' | -40.9 | NC_003663.2 | + | 3949 | 0.67 | 1 |
Target: 5'- aGUUgCUAccucauuAAGAACGGAGAAgUAUCc- -3' miRNA: 3'- aCAAaGAU-------UUCUUGCCUCUU-AUAGau -5' |
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14927 | 3' | -40.9 | NC_003663.2 | + | 59916 | 0.67 | 1 |
Target: 5'- cGUcaUUCUAAAaGACGGAGGAUcuccAUCa- -3' miRNA: 3'- aCA--AAGAUUUcUUGCCUCUUA----UAGau -5' |
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14927 | 3' | -40.9 | NC_003663.2 | + | 176695 | 0.67 | 1 |
Target: 5'- cGggUCauguGAcGACGGAGAAUAUCUu -3' miRNA: 3'- aCaaAGau--UUcUUGCCUCUUAUAGAu -5' |
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14927 | 3' | -40.9 | NC_003663.2 | + | 145029 | 0.67 | 1 |
Target: 5'- --aUUCUuugguAGAAUGGGGAAcAUCUAc -3' miRNA: 3'- acaAAGAuu---UCUUGCCUCUUaUAGAU- -5' |
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14927 | 3' | -40.9 | NC_003663.2 | + | 220551 | 0.67 | 1 |
Target: 5'- aGUUgCUAccucauuAAGAACGGAGAAgUAUCc- -3' miRNA: 3'- aCAAaGAU-------UUCUUGCCUCUU-AUAGau -5' |
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14927 | 3' | -40.9 | NC_003663.2 | + | 70981 | 0.77 | 0.998959 |
Target: 5'- aUGUUgUCUGAAGGACGGAuGAAU-UCUAa -3' miRNA: 3'- -ACAA-AGAUUUCUUGCCU-CUUAuAGAU- -5' |
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14927 | 3' | -40.9 | NC_003663.2 | + | 70692 | 1.09 | 0.123725 |
Target: 5'- cUGUUUCUAAAGAACGGAGAAUAUCUAu -3' miRNA: 3'- -ACAAAGAUUUCUUGCCUCUUAUAGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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