Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1495 | 5' | -55.8 | NC_001335.1 | + | 48050 | 0.66 | 0.650978 |
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Target: 5'- gGUGUcGAcugcacCGUCGCCGAcagcGGaccaGUGCUCg -3' miRNA: 3'- -UACAuCUu-----GCAGCGGCU----CCg---CACGAG- -5' |
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| 1495 | 5' | -55.8 | NC_001335.1 | + | 29167 | 0.66 | 0.61649 |
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Target: 5'- -gGUGGGGCGaaggcucUCGCUGAGGCaGUGgggauCUCg -3' miRNA: 3'- uaCAUCUUGC-------AGCGGCUCCG-CAC-----GAG- -5' |
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| 1495 | 5' | -55.8 | NC_001335.1 | + | 45611 | 0.67 | 0.595399 |
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Target: 5'- -gGUcgcuGAcCGUCGaguaCGAGGaCGUGCUCg -3' miRNA: 3'- uaCAu---CUuGCAGCg---GCUCC-GCACGAG- -5' |
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| 1495 | 5' | -55.8 | NC_001335.1 | + | 41523 | 0.68 | 0.519133 |
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Target: 5'- gAUGUAGAGCGaUUGUCuAGGUGUGUa- -3' miRNA: 3'- -UACAUCUUGC-AGCGGcUCCGCACGag -5' |
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| 1495 | 5' | -55.8 | NC_001335.1 | + | 12758 | 0.68 | 0.508526 |
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Target: 5'- ---cGGAACGUCGCCGAcgcaGCG-GCUUu -3' miRNA: 3'- uacaUCUUGCAGCGGCUc---CGCaCGAG- -5' |
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| 1495 | 5' | -55.8 | NC_001335.1 | + | 28092 | 0.69 | 0.447019 |
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Target: 5'- ---gGGGACGUUGUCGGGGCGUucgGCg- -3' miRNA: 3'- uacaUCUUGCAGCGGCUCCGCA---CGag -5' |
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| 1495 | 5' | -55.8 | NC_001335.1 | + | 32034 | 1.08 | 0.00081 |
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Target: 5'- gAUGUAGAACGUCGCCGAGGCGUGCUCg -3' miRNA: 3'- -UACAUCUUGCAGCGGCUCCGCACGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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