miRNA: 3'- -UAGAGGGUGUGA------AGCCuGUCCAgAga -5'
miRNA: 3'- -UAGAGGGUGUGAAGCCUGUCCAGAGA- -5'
Results 1 - 2 of 2 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14951 | 3' | -54.9 | NC_003679.1 | + | 3085 | 0.66 | 0.291729 |
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Target: 5'- gGUCUCCCACACUauccaaUUGG-UAGGUgUa- -3' miRNA: 3'- -UAGAGGGUGUGA------AGCCuGUCCAgAga -5' |
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| 14951 | 3' | -54.9 | NC_003679.1 | + | 4887 | 1.05 | 0.000229 |
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Target: 5'- aAUCUCCCACACUUCGGACAGGUCUCUc -3' miRNA: 3'- -UAGAGGGUGUGAAGCCUGUCCAGAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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