Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14957 | 3' | -42.3 | NC_003687.1 | + | 9830 | 0.66 | 0.957275 |
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Target: 5'- aCCAAgaugagcugguUGGCAGGGca-AGGguGUCACCa -3' miRNA: 3'- -GGUU-----------AUCGUUCUucaUUUguCAGUGG- -5' |
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| 14957 | 3' | -42.3 | NC_003687.1 | + | 7624 | 0.67 | 0.934081 |
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Target: 5'- cCCAGggAGCuAGAAGgggugGAGCAGagggaagCACCc -3' miRNA: 3'- -GGUUa-UCGuUCUUCa----UUUGUCa------GUGG- -5' |
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| 14957 | 3' | -42.3 | NC_003687.1 | + | 7431 | 0.67 | 0.934081 |
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Target: 5'- gCCGAUGGCGAGAAacAAAC--UCAUCu -3' miRNA: 3'- -GGUUAUCGUUCUUcaUUUGucAGUGG- -5' |
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| 14957 | 3' | -42.3 | NC_003687.1 | + | 935 | 0.68 | 0.912288 |
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Target: 5'- cUCAAcgcCAAGAGGauGACAGUCACUc -3' miRNA: 3'- -GGUUaucGUUCUUCauUUGUCAGUGG- -5' |
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| 14957 | 3' | -42.3 | NC_003687.1 | + | 7232 | 0.75 | 0.551694 |
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Target: 5'- cCCAAU-GCAagGGAGGUGGGC-GUCGCUc -3' miRNA: 3'- -GGUUAuCGU--UCUUCAUUUGuCAGUGG- -5' |
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| 14957 | 3' | -42.3 | NC_003687.1 | + | 3846 | 1.2 | 0.000738 |
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Target: 5'- aCCAAUAGCAAGAAGUAAACAGUCACCa -3' miRNA: 3'- -GGUUAUCGUUCUUCAUUUGUCAGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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