miRNA: 3'- gaCGGUUAccGUAGa-GUCCCGCUGAG- -5'
miRNA: 3'- gACGGUuACCGU-AGaGUCCCGCUGag -5'
miRNA: 3'- -GACGGUUACCGUAGAGUCCCGCUGAG- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14961 | 5' | -55.6 | NC_003687.1 | + | 3446 | 0.66 | 0.21921 |
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Target: 5'- -gGCCAGUc-CAUUcgCAGGGCGGCUUg -3' miRNA: 3'- gaCGGUUAccGUAGa-GUCCCGCUGAG- -5' |
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| 14961 | 5' | -55.6 | NC_003687.1 | + | 5108 | 0.74 | 0.057098 |
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Target: 5'- aUGCCAuUGGCAgUC-CAGGGUGGCa- -3' miRNA: 3'- gACGGUuACCGU-AGaGUCCCGCUGag -5' |
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| 14961 | 5' | -55.6 | NC_003687.1 | + | 5131 | 1.1 | 6.1e-05 |
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Target: 5'- aCUGCCAAUGGCAUCUCAGGGCGACUCu -3' miRNA: 3'- -GACGGUUACCGUAGAGUCCCGCUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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