Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14967 | 5' | -47.5 | NC_003687.1 | + | 314 | 0.66 | 0.680699 |
Target: 5'- aGGCGGAGGcuGUUCUGAaGAAGAUa-- -3' miRNA: 3'- -CCGCCUUUu-UAGGGCUcCUUCUAacg -5' |
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14967 | 5' | -47.5 | NC_003687.1 | + | 6270 | 0.67 | 0.667749 |
Target: 5'- -cUGGGAAGGUCCCGAcG-AGAaUGCc -3' miRNA: 3'- ccGCCUUUUUAGGGCUcCuUCUaACG- -5' |
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14967 | 5' | -47.5 | NC_003687.1 | + | 6563 | 0.75 | 0.241612 |
Target: 5'- uGGCGGAGAGAgaugcUCCUGAGGc-GAU-GCu -3' miRNA: 3'- -CCGCCUUUUU-----AGGGCUCCuuCUAaCG- -5' |
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14967 | 5' | -47.5 | NC_003687.1 | + | 10720 | 1.15 | 0.000306 |
Target: 5'- aGGCGGAAAAAUCCCGAGGAAGAUUGCc -3' miRNA: 3'- -CCGCCUUUUUAGGGCUCCUUCUAACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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