Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14969 | 3' | -46.9 | NC_003687.1 | + | 3642 | 0.66 | 0.698698 |
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Target: 5'- cGUG-AGAGUGGAGGAGaUCGUgCGCu- -3' miRNA: 3'- -CACuUCUUACCUUUUC-GGUAgGUGug -5' |
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| 14969 | 3' | -46.9 | NC_003687.1 | + | 2227 | 0.67 | 0.618629 |
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Target: 5'- -cGggGuacugucuucuGUGGGGAAGgCaAUCCACACg -3' miRNA: 3'- caCuuCu----------UACCUUUUCgG-UAGGUGUG- -5' |
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| 14969 | 3' | -46.9 | NC_003687.1 | + | 4933 | 0.69 | 0.526161 |
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Target: 5'- uUGAGGGggGGAGAguucugggGGCCAUCC-CGa -3' miRNA: 3'- cACUUCUuaCCUUU--------UCGGUAGGuGUg -5' |
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| 14969 | 3' | -46.9 | NC_003687.1 | + | 7937 | 0.69 | 0.522296 |
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Target: 5'- uGUGAgagcuuacaccauuGGAG-GGAAGGGCCAUgaagcaCCACGCc -3' miRNA: 3'- -CACU--------------UCUUaCCUUUUCGGUA------GGUGUG- -5' |
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| 14969 | 3' | -46.9 | NC_003687.1 | + | 1655 | 0.69 | 0.488046 |
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Target: 5'- uGUGGAGAaacuuGUGGAAuuuGGaCCA-CCACAUg -3' miRNA: 3'- -CACUUCU-----UACCUUu--UC-GGUaGGUGUG- -5' |
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| 14969 | 3' | -46.9 | NC_003687.1 | + | 2997 | 1.12 | 0.000425 |
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Target: 5'- cGUGAAGAAUGGAAAAGCCAUCCACACg -3' miRNA: 3'- -CACUUCUUACCUUUUCGGUAGGUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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