miRNA: 3'- -GGguCGua-CGCGuaGUCAUGuUCAGG- -5'
miRNA: 3'- -GGGU--CGuaCGCGUAGUCAuGUucAGG- -5'
miRNA: 3'- gGgUCGuACGCGUAGUCaUGUUCa--GG- -5'
miRNA: 3'- -GGGUcgua-C-GCGUAGUC-AUGUUCAGG- -5'
miRNA: 3'- -GGGUCGUACGCGUAGUCAUGUUCAGG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14976 | 3' | -50.9 | NC_003690.1 | + | 8550 | 0.66 | 0.46728 |
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Target: 5'- cUCguGCcagGUGUcgCAGUACuGGUCCu -3' miRNA: 3'- -GGguCGua-CGCGuaGUCAUGuUCAGG- -5' |
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| 14976 | 3' | -50.9 | NC_003690.1 | + | 3935 | 0.68 | 0.3792 |
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Target: 5'- cCCCAucGCcaGCGCAUCAGUcCAucUCCa -3' miRNA: 3'- -GGGU--CGuaCGCGUAGUCAuGUucAGG- -5' |
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| 14976 | 3' | -50.9 | NC_003690.1 | + | 4411 | 0.69 | 0.320855 |
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Target: 5'- gUgAGCcUGCGCGUCAG-ACAAGaugCCc -3' miRNA: 3'- gGgUCGuACGCGUAGUCaUGUUCa--GG- -5' |
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| 14976 | 3' | -50.9 | NC_003690.1 | + | 195 | 0.7 | 0.269495 |
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Target: 5'- cCCCAaagaaGaCGCGUCAGuUGCGGGUCCa -3' miRNA: 3'- -GGGUcgua-C-GCGUAGUC-AUGUUCAGG- -5' |
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| 14976 | 3' | -50.9 | NC_003690.1 | + | 272 | 1.15 | 0.000101 |
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Target: 5'- uCCCAGCAUGCGCAUCAGUACAAGUCCa -3' miRNA: 3'- -GGGUCGUACGCGUAGUCAUGUUCAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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