Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14981 | 5' | -48.6 | NC_003690.1 | + | 3000 | 0.67 | 0.500588 |
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Target: 5'- gGGUCAU---GGGGGcuGCGGUCAAGa -3' miRNA: 3'- aCCGGUGuguUUCUUuuCGCCAGUUC- -5' |
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| 14981 | 5' | -48.6 | NC_003690.1 | + | 5960 | 0.68 | 0.464007 |
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Target: 5'- -uGCUGCACAGcgcaggGGAAGAGUGGgcagCAGGg -3' miRNA: 3'- acCGGUGUGUU------UCUUUUCGCCa---GUUC- -5' |
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| 14981 | 5' | -48.6 | NC_003690.1 | + | 4057 | 0.7 | 0.373971 |
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Target: 5'- aUGGCCAUAUGGAGAuacaucAGgGGcCAGGg -3' miRNA: 3'- -ACCGGUGUGUUUCUuu----UCgCCaGUUC- -5' |
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| 14981 | 5' | -48.6 | NC_003690.1 | + | 6753 | 0.7 | 0.343396 |
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Target: 5'- cGGcCCACAgGAAGAucaguGAGGUGG-CGAGa -3' miRNA: 3'- aCC-GGUGUgUUUCU-----UUUCGCCaGUUC- -5' |
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| 14981 | 5' | -48.6 | NC_003690.1 | + | 7561 | 0.72 | 0.262747 |
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Target: 5'- gUGGCCugugGCAuGAGugGAGUGGUCAGGg -3' miRNA: 3'- -ACCGGug--UGU-UUCuuUUCGCCAGUUC- -5' |
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| 14981 | 5' | -48.6 | NC_003690.1 | + | 7860 | 0.72 | 0.25402 |
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Target: 5'- gGGCUACGuCAcccugaaAGGGGAAGUGGUCGAc -3' miRNA: 3'- aCCGGUGU-GU-------UUCUUUUCGCCAGUUc -5' |
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| 14981 | 5' | -48.6 | NC_003690.1 | + | 4062 | 1.09 | 0.000485 |
|
Target: 5'- gUGGCCACACAAAGAAAAGCGGUCAAGc -3' miRNA: 3'- -ACCGGUGUGUUUCUUUUCGCCAGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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