Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14984 | 5' | -51.9 | NC_003690.1 | + | 9926 | 0.66 | 0.387108 |
Target: 5'- aCAGGCCgucuCCcgcacacuCCAGCCGc--AGCCAg -3' miRNA: 3'- -GUUCGGau--GGu-------GGUCGGUuuuUCGGU- -5' |
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14984 | 5' | -51.9 | NC_003690.1 | + | 4046 | 0.66 | 0.38394 |
Target: 5'- -uGGCC-ACUACCauGGCCAuauggagauacaucAGGGGCCAg -3' miRNA: 3'- guUCGGaUGGUGG--UCGGU--------------UUUUCGGU- -5' |
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14984 | 5' | -51.9 | NC_003690.1 | + | 7601 | 0.66 | 0.366318 |
Target: 5'- gGGGCCUucuGCCACuCGGCCAcAGAcuguGGCUc -3' miRNA: 3'- gUUCGGA---UGGUG-GUCGGU-UUU----UCGGu -5' |
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14984 | 5' | -51.9 | NC_003690.1 | + | 6792 | 0.67 | 0.336604 |
Target: 5'- cCAGuGCC-ACC-CCAGCCAuguucccguAGCCAa -3' miRNA: 3'- -GUU-CGGaUGGuGGUCGGUuuu------UCGGU- -5' |
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14984 | 5' | -51.9 | NC_003690.1 | + | 3230 | 0.67 | 0.327098 |
Target: 5'- gUAGGUUgACCuagguCCAGCCAGAcuGGCCGg -3' miRNA: 3'- -GUUCGGaUGGu----GGUCGGUUUu-UCGGU- -5' |
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14984 | 5' | -51.9 | NC_003690.1 | + | 4209 | 0.67 | 0.327098 |
Target: 5'- --cGUCUcccccuggagGCCGCCAacucCCAGAAGGCCAg -3' miRNA: 3'- guuCGGA----------UGGUGGUc---GGUUUUUCGGU- -5' |
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14984 | 5' | -51.9 | NC_003690.1 | + | 7300 | 0.67 | 0.299781 |
Target: 5'- --uGCCUccagucccgacACCACUauGGCCAggugGAAAGCCGc -3' miRNA: 3'- guuCGGA-----------UGGUGG--UCGGU----UUUUCGGU- -5' |
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14984 | 5' | -51.9 | NC_003690.1 | + | 1684 | 0.68 | 0.28257 |
Target: 5'- cCAAGCCUcagcuCCAUCAuGCUuccaagGGAGGGCCAg -3' miRNA: 3'- -GUUCGGAu----GGUGGU-CGG------UUUUUCGGU- -5' |
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14984 | 5' | -51.9 | NC_003690.1 | + | 6909 | 0.68 | 0.28257 |
Target: 5'- -cAGCC-ACCAucCCGGCCA---GGCCGc -3' miRNA: 3'- guUCGGaUGGU--GGUCGGUuuuUCGGU- -5' |
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14984 | 5' | -51.9 | NC_003690.1 | + | 6155 | 0.69 | 0.242982 |
Target: 5'- gCAGGCCacUACCGCCuGCCAGAGGa--- -3' miRNA: 3'- -GUUCGG--AUGGUGGuCGGUUUUUcggu -5' |
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14984 | 5' | -51.9 | NC_003690.1 | + | 3175 | 0.69 | 0.242982 |
Target: 5'- aAAGCUguuuCUACCGGCCAGucuGGCUg -3' miRNA: 3'- gUUCGGau--GGUGGUCGGUUuu-UCGGu -5' |
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14984 | 5' | -51.9 | NC_003690.1 | + | 4520 | 1.08 | 0.000194 |
Target: 5'- uCAAGCCUACCACCAGCCAAAAAGCCAu -3' miRNA: 3'- -GUUCGGAUGGUGGUCGGUUUUUCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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