Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14989 | 5' | -54.7 | NC_003690.1 | + | 1177 | 0.66 | 0.28101 |
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Target: 5'- gUGGCUGCCagaugcccgacaauGGGGCCAGcCACGcuuccagaggaGCACCa -3' miRNA: 3'- -ACUGGUGG--------------CUCCGGUUcGUGU-----------UGUGG- -5' |
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| 14989 | 5' | -54.7 | NC_003690.1 | + | 4543 | 0.69 | 0.17055 |
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Target: 5'- cGACCagugaaagGCCGAGGCaauCAAGCcUAcCACCa -3' miRNA: 3'- aCUGG--------UGGCUCCG---GUUCGuGUuGUGG- -5' |
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| 14989 | 5' | -54.7 | NC_003690.1 | + | 7208 | 0.69 | 0.170023 |
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Target: 5'- cGAUC-CCGAGuGCCAAGaCAUGucccgccACACCa -3' miRNA: 3'- aCUGGuGGCUC-CGGUUC-GUGU-------UGUGG- -5' |
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| 14989 | 5' | -54.7 | NC_003690.1 | + | 9877 | 0.7 | 0.150593 |
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Target: 5'- cUGcCCACCaacucauccuGGUCAcGGCACGGCACCa -3' miRNA: 3'- -ACuGGUGGcu--------CCGGU-UCGUGUUGUGG- -5' |
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| 14989 | 5' | -54.7 | NC_003690.1 | + | 4315 | 0.7 | 0.145947 |
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Target: 5'- -aGCCGCC-AGcGCCAuGGCAC-ACACCg -3' miRNA: 3'- acUGGUGGcUC-CGGU-UCGUGuUGUGG- -5' |
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| 14989 | 5' | -54.7 | NC_003690.1 | + | 7470 | 1.13 | 0.000052 |
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Target: 5'- aUGACCACCGAGGCCAAGCACAACACCa -3' miRNA: 3'- -ACUGGUGGCUCCGGUUCGUGUUGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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