Results 1 - 20 of 40 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1499 | 3' | -54.2 | NC_001335.1 | + | 51788 | 0.66 | 0.802429 |
Target: 5'- aCUAcGUGCccggUAUCGGCGUugUCGUGCCg -3' miRNA: 3'- gGGU-CACGua--GUAGCUGUA--GGCGCGG- -5' |
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1499 | 3' | -54.2 | NC_001335.1 | + | 32168 | 0.66 | 0.802429 |
Target: 5'- aCUCGGccaGC-UCGUCGGCAUCgaaGuCGCCg -3' miRNA: 3'- -GGGUCa--CGuAGUAGCUGUAGg--C-GCGG- -5' |
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1499 | 3' | -54.2 | NC_001335.1 | + | 8944 | 0.66 | 0.802429 |
Target: 5'- gCCGaUGgAgacCAUCGGCugguUCCGCGCUg -3' miRNA: 3'- gGGUcACgUa--GUAGCUGu---AGGCGCGG- -5' |
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1499 | 3' | -54.2 | NC_001335.1 | + | 7557 | 0.66 | 0.79664 |
Target: 5'- uUCCAGcGCGUCguacaucaucccgguGUCGACAgagUCGCcuGCCu -3' miRNA: 3'- -GGGUCaCGUAG---------------UAGCUGUa--GGCG--CGG- -5' |
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1499 | 3' | -54.2 | NC_001335.1 | + | 47977 | 0.66 | 0.782893 |
Target: 5'- aCCAGggagGCGUgAcCGGCAaguUCUGgGCCa -3' miRNA: 3'- gGGUCa---CGUAgUaGCUGU---AGGCgCGG- -5' |
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1499 | 3' | -54.2 | NC_001335.1 | + | 43913 | 0.66 | 0.782893 |
Target: 5'- gCUCAGUGUcgCuGUCGACGgg-GCGCUg -3' miRNA: 3'- -GGGUCACGuaG-UAGCUGUaggCGCGG- -5' |
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1499 | 3' | -54.2 | NC_001335.1 | + | 1530 | 0.66 | 0.772885 |
Target: 5'- aCCUugaugGCGUCGUgGugAUCCGgGUCc -3' miRNA: 3'- -GGGuca--CGUAGUAgCugUAGGCgCGG- -5' |
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1499 | 3' | -54.2 | NC_001335.1 | + | 45509 | 0.66 | 0.771876 |
Target: 5'- aCCAGUGgAagAcgUGGCAUgacgagcCCGCGCCg -3' miRNA: 3'- gGGUCACgUagUa-GCUGUA-------GGCGCGG- -5' |
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1499 | 3' | -54.2 | NC_001335.1 | + | 26690 | 0.66 | 0.76273 |
Target: 5'- uCUCGGggGCAUCAgcgCGGuCcUCCGUGCUu -3' miRNA: 3'- -GGGUCa-CGUAGUa--GCU-GuAGGCGCGG- -5' |
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1499 | 3' | -54.2 | NC_001335.1 | + | 19104 | 0.66 | 0.76273 |
Target: 5'- gCgAGaacUGCGUCAUCGACAccgaCCgGCGCg -3' miRNA: 3'- gGgUC---ACGUAGUAGCUGUa---GG-CGCGg -5' |
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1499 | 3' | -54.2 | NC_001335.1 | + | 47982 | 0.67 | 0.74203 |
Target: 5'- -aCGGUGCA--GUCGACA-CCaGCGCa -3' miRNA: 3'- ggGUCACGUagUAGCUGUaGG-CGCGg -5' |
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1499 | 3' | -54.2 | NC_001335.1 | + | 29327 | 0.67 | 0.74203 |
Target: 5'- aUCCAGUcCGaCGaCGGCAgcgUCUGCGCCg -3' miRNA: 3'- -GGGUCAcGUaGUaGCUGU---AGGCGCGG- -5' |
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1499 | 3' | -54.2 | NC_001335.1 | + | 34291 | 0.67 | 0.731507 |
Target: 5'- gCUCAGUGUGUUGUggcCGGCGgUgUGCGCCg -3' miRNA: 3'- -GGGUCACGUAGUA---GCUGU-AgGCGCGG- -5' |
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1499 | 3' | -54.2 | NC_001335.1 | + | 15650 | 0.67 | 0.720885 |
Target: 5'- gCCUGGaugGCGUCGUaGAUGUCCGCGa- -3' miRNA: 3'- -GGGUCa--CGUAGUAgCUGUAGGCGCgg -5' |
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1499 | 3' | -54.2 | NC_001335.1 | + | 5369 | 0.67 | 0.710174 |
Target: 5'- uUCGGUGCAgcUCAcCGAgAUCgccugcgcgagCGCGCCg -3' miRNA: 3'- gGGUCACGU--AGUaGCUgUAG-----------GCGCGG- -5' |
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1499 | 3' | -54.2 | NC_001335.1 | + | 13603 | 0.67 | 0.699387 |
Target: 5'- uCUCGGacgGCGUCccucgucUCGACGUaCCGgGCCg -3' miRNA: 3'- -GGGUCa--CGUAGu------AGCUGUA-GGCgCGG- -5' |
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1499 | 3' | -54.2 | NC_001335.1 | + | 6496 | 0.67 | 0.699387 |
Target: 5'- gCCAGaGcCAUCAUCGACGcccucacguUCUuCGCCa -3' miRNA: 3'- gGGUCaC-GUAGUAGCUGU---------AGGcGCGG- -5' |
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1499 | 3' | -54.2 | NC_001335.1 | + | 14195 | 0.68 | 0.688533 |
Target: 5'- aUCCAGgGUcugAUCAggcUCGGCGUCCGCaGaCCg -3' miRNA: 3'- -GGGUCaCG---UAGU---AGCUGUAGGCG-C-GG- -5' |
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1499 | 3' | -54.2 | NC_001335.1 | + | 4604 | 0.68 | 0.677626 |
Target: 5'- gCgCAGUGgGUCcaCGACAUggcCCGCGCUc -3' miRNA: 3'- -GgGUCACgUAGuaGCUGUA---GGCGCGG- -5' |
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1499 | 3' | -54.2 | NC_001335.1 | + | 8590 | 0.68 | 0.677626 |
Target: 5'- cCCCAG-GCucuaCGUCGACucaGUCgccgaGCGCCa -3' miRNA: 3'- -GGGUCaCGua--GUAGCUG---UAGg----CGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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