Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14990 | 5' | -55.1 | NC_003690.1 | + | 4388 | 0.66 | 0.269573 |
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Target: 5'- aCUCaaUGuucCCACUCCACUCA-GCCaACAa -3' miRNA: 3'- -GGG--ACu--GGUGAGGUGAGUaCGG-UGUc -5' |
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| 14990 | 5' | -55.1 | NC_003690.1 | + | 6162 | 0.67 | 0.226186 |
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Target: 5'- uCUCUGucauCCuCUCCuGCUCAggGCCAUAGa -3' miRNA: 3'- -GGGACu---GGuGAGG-UGAGUa-CGGUGUC- -5' |
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| 14990 | 5' | -55.1 | NC_003690.1 | + | 3045 | 0.68 | 0.177682 |
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Target: 5'- ---cGGCCAUUCCAUUCuUGaCCGCAGc -3' miRNA: 3'- gggaCUGGUGAGGUGAGuAC-GGUGUC- -5' |
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| 14990 | 5' | -55.1 | NC_003690.1 | + | 7595 | 0.71 | 0.118406 |
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Target: 5'- gCCUguggGGCCuuCUgCCACUCG-GCCACAGa -3' miRNA: 3'- -GGGa---CUGGu-GA-GGUGAGUaCGGUGUC- -5' |
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| 14990 | 5' | -55.1 | NC_003690.1 | + | 2671 | 0.71 | 0.113613 |
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Target: 5'- uCCacaUGGCCAuCUCCAgUCuauuuugagggacaAUGCCACAGa -3' miRNA: 3'- -GGg--ACUGGU-GAGGUgAG--------------UACGGUGUC- -5' |
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| 14990 | 5' | -55.1 | NC_003690.1 | + | 7602 | 1.11 | 6.5e-05 |
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Target: 5'- cCCCUGACCACUCCACUCAUGCCACAGg -3' miRNA: 3'- -GGGACUGGUGAGGUGAGUACGGUGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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