Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1501 | 3' | -48.6 | NC_001335.1 | + | 19452 | 0.66 | 0.960237 |
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Target: 5'- cGGCcUGGUCG-CuGGCGAGCGGGugcUCg -3' miRNA: 3'- -UUGaACCAGUaGuCUGCUUGCUCu--AG- -5' |
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| 1501 | 3' | -48.6 | NC_001335.1 | + | 44391 | 0.66 | 0.956013 |
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Target: 5'- gAACUccucUGGUCGUggcCAGACGAcauCGAGGa- -3' miRNA: 3'- -UUGA----ACCAGUA---GUCUGCUu--GCUCUag -5' |
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| 1501 | 3' | -48.6 | NC_001335.1 | + | 24349 | 0.67 | 0.941022 |
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Target: 5'- -cCUUGGUCGUCGcaccugccugagcGGCGAACGuAGcgCc -3' miRNA: 3'- uuGAACCAGUAGU-------------CUGCUUGC-UCuaG- -5' |
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| 1501 | 3' | -48.6 | NC_001335.1 | + | 33776 | 0.68 | 0.917948 |
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Target: 5'- -----cGUCAUCAGACGAuGCGcccGGAUCa -3' miRNA: 3'- uugaacCAGUAGUCUGCU-UGC---UCUAG- -5' |
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| 1501 | 3' | -48.6 | NC_001335.1 | + | 30925 | 0.68 | 0.902122 |
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Target: 5'- ----gGGUCAUuaguggugauccccCGGACGGugcGCGAGAUCg -3' miRNA: 3'- uugaaCCAGUA--------------GUCUGCU---UGCUCUAG- -5' |
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| 1501 | 3' | -48.6 | NC_001335.1 | + | 42100 | 0.69 | 0.881495 |
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Target: 5'- gGACUcGGUCGUCGGcaaGGACGAcacGGUCu -3' miRNA: 3'- -UUGAaCCAGUAGUCug-CUUGCU---CUAG- -5' |
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| 1501 | 3' | -48.6 | NC_001335.1 | + | 27025 | 0.72 | 0.733837 |
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Target: 5'- uGACgaugGGUCucgUAGGCGAagccgGCGAGAUCg -3' miRNA: 3'- -UUGaa--CCAGua-GUCUGCU-----UGCUCUAG- -5' |
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| 1501 | 3' | -48.6 | NC_001335.1 | + | 34810 | 1.08 | 0.004743 |
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Target: 5'- gAACUUGGUCAUCAGACGAACGAGAUCu -3' miRNA: 3'- -UUGAACCAGUAGUCUGCUUGCUCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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