Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1503 | 5' | -55.3 | NC_001335.1 | + | 4684 | 0.66 | 0.690114 |
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Target: 5'- -aGCGCGGgccaUGUC-GUGGACccaCUGCGc -3' miRNA: 3'- caUGCGCC----ACAGuCGCCUGaa-GACGC- -5' |
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| 1503 | 5' | -55.3 | NC_001335.1 | + | 48320 | 0.66 | 0.690114 |
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Target: 5'- -gGCGUGG-GcCAGCaGGACguggccgCUGCGg -3' miRNA: 3'- caUGCGCCaCaGUCG-CCUGaa-----GACGC- -5' |
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| 1503 | 5' | -55.3 | NC_001335.1 | + | 44173 | 0.67 | 0.657173 |
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Target: 5'- --uUGCGGUGagGuGUGGACgagguugUCUGCGa -3' miRNA: 3'- cauGCGCCACagU-CGCCUGa------AGACGC- -5' |
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| 1503 | 5' | -55.3 | NC_001335.1 | + | 22822 | 0.67 | 0.620703 |
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Target: 5'- cUGCGgGGUuggugcggacgaacGUC-GCGGugUUCUGCu -3' miRNA: 3'- cAUGCgCCA--------------CAGuCGCCugAAGACGc -5' |
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| 1503 | 5' | -55.3 | NC_001335.1 | + | 25789 | 0.68 | 0.578861 |
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Target: 5'- uUGCaGCGGUcggaacccucgccGUCGGCGGGCUg--GCGu -3' miRNA: 3'- cAUG-CGCCA-------------CAGUCGCCUGAagaCGC- -5' |
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| 1503 | 5' | -55.3 | NC_001335.1 | + | 36513 | 1.08 | 0.001096 |
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Target: 5'- uGUACGCGGUGUCAGCGGACUUCUGCGu -3' miRNA: 3'- -CAUGCGCCACAGUCGCCUGAAGACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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