Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1504 | 5' | -54.2 | NC_001335.1 | + | 1872 | 0.66 | 0.755898 |
Target: 5'- cCGGGgaauacGUCCcagcucgacAUCAGcgaGUCGCCGAUGg -3' miRNA: 3'- -GCCCau----CAGG---------UAGUC---CAGUGGCUACa -5' |
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1504 | 5' | -54.2 | NC_001335.1 | + | 14819 | 0.66 | 0.745452 |
Target: 5'- aCGGGcAG-CgCAgcCAGGUCAUCGAUGg -3' miRNA: 3'- -GCCCaUCaG-GUa-GUCCAGUGGCUACa -5' |
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1504 | 5' | -54.2 | NC_001335.1 | + | 13216 | 0.67 | 0.71346 |
Target: 5'- gGGGUAGaCgAUgCAGGUCugguaccgggcACCGGUGUc -3' miRNA: 3'- gCCCAUCaGgUA-GUCCAG-----------UGGCUACA- -5' |
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1504 | 5' | -54.2 | NC_001335.1 | + | 4996 | 0.69 | 0.581162 |
Target: 5'- gGGGUGGUCCAUC--GUCAUcaacgugcaagCGGUGUg -3' miRNA: 3'- gCCCAUCAGGUAGucCAGUG-----------GCUACA- -5' |
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1504 | 5' | -54.2 | NC_001335.1 | + | 36861 | 1.08 | 0.001496 |
Target: 5'- uCGGGUAGUCCAUCAGGUCACCGAUGUg -3' miRNA: 3'- -GCCCAUCAGGUAGUCCAGUGGCUACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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