miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1504 5' -54.2 NC_001335.1 + 1872 0.66 0.755898
Target:  5'- cCGGGgaauacGUCCcagcucgacAUCAGcgaGUCGCCGAUGg -3'
miRNA:   3'- -GCCCau----CAGG---------UAGUC---CAGUGGCUACa -5'
1504 5' -54.2 NC_001335.1 + 14819 0.66 0.745452
Target:  5'- aCGGGcAG-CgCAgcCAGGUCAUCGAUGg -3'
miRNA:   3'- -GCCCaUCaG-GUa-GUCCAGUGGCUACa -5'
1504 5' -54.2 NC_001335.1 + 13216 0.67 0.71346
Target:  5'- gGGGUAGaCgAUgCAGGUCugguaccgggcACCGGUGUc -3'
miRNA:   3'- gCCCAUCaGgUA-GUCCAG-----------UGGCUACA- -5'
1504 5' -54.2 NC_001335.1 + 4996 0.69 0.581162
Target:  5'- gGGGUGGUCCAUC--GUCAUcaacgugcaagCGGUGUg -3'
miRNA:   3'- gCCCAUCAGGUAGucCAGUG-----------GCUACA- -5'
1504 5' -54.2 NC_001335.1 + 36861 1.08 0.001496
Target:  5'- uCGGGUAGUCCAUCAGGUCACCGAUGUg -3'
miRNA:   3'- -GCCCAUCAGGUAGUCCAGUGGCUACA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.