miRNA: 3'- -GGCGg--ACCCCUcuCaACU----AGUCGCGU- -5'
miRNA: 3'- gGCGGACCCCucUCaACUAGuc-GCGU- -5'
miRNA: 3'- -GGCGGAC-CCCUcUCAacu----AGUCGCGu -5'
miRNA: 3'- gGCGGAcCC-CUCuCAACU--AGUCGCGU- -5'
miRNA: 3'- -GGCGGACCCCUCUCAACUAGUCGCGU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1509 | 5' | -57 | NC_001335.1 | + | 25388 | 0.66 | 0.621869 |
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Target: 5'- gUCGCggaUGGGGAucGcUGAagacUCAGCGCAg -3' miRNA: 3'- -GGCGg--ACCCCUcuCaACU----AGUCGCGU- -5' |
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| 1509 | 5' | -57 | NC_001335.1 | + | 41672 | 0.67 | 0.546088 |
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Target: 5'- aCGCCUGGGGccGGcUGGUCucuCGCGa -3' miRNA: 3'- gGCGGACCCCucUCaACUAGuc-GCGU- -5' |
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| 1509 | 5' | -57 | NC_001335.1 | + | 28579 | 0.67 | 0.546088 |
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Target: 5'- gUCGCCUGcGGGAaGGUggcgcacUCGGCGCu -3' miRNA: 3'- -GGCGGAC-CCCUcUCAacu----AGUCGCGu -5' |
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| 1509 | 5' | -57 | NC_001335.1 | + | 27287 | 0.7 | 0.396948 |
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Target: 5'- aCGCCUcGGcGAG-GUUGGccUCGGCGCGg -3' miRNA: 3'- gGCGGAcCC-CUCuCAACU--AGUCGCGU- -5' |
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| 1509 | 5' | -57 | NC_001335.1 | + | 40310 | 1.1 | 0.000497 |
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Target: 5'- cCCGCCUGGGGAGAGUUGAUCAGCGCAg -3' miRNA: 3'- -GGCGGACCCCUCUCAACUAGUCGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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