Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 151 | 3' | -56.4 | AC_000006.1 | + | 11075 | 0.66 | 0.528578 |
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Target: 5'- uGCGCU----CCUGCuUCACCGUCUGc -3' miRNA: 3'- uCGCGAgcauGGGCG-AGUGGUAGACu -5' |
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| 151 | 3' | -56.4 | AC_000006.1 | + | 6248 | 0.67 | 0.46377 |
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Target: 5'- uGGCGCgucaggaUCGUGCCCGaCgagcgcacCACCGUCUu- -3' miRNA: 3'- -UCGCG-------AGCAUGGGC-Ga-------GUGGUAGAcu -5' |
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| 151 | 3' | -56.4 | AC_000006.1 | + | 9092 | 0.67 | 0.424579 |
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Target: 5'- gAGCGCgagGUGCgCGC-CACCAUCgccGAg -3' miRNA: 3'- -UCGCGag-CAUGgGCGaGUGGUAGa--CU- -5' |
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| 151 | 3' | -56.4 | AC_000006.1 | + | 24080 | 0.67 | 0.424579 |
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Target: 5'- uGCGCUCGUcaaaGCCCGUcCACagcuuUUUGAg -3' miRNA: 3'- uCGCGAGCA----UGGGCGaGUGgu---AGACU- -5' |
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| 151 | 3' | -56.4 | AC_000006.1 | + | 15609 | 0.68 | 0.377352 |
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Target: 5'- gGGCGUgcgCGUGCCCGUgcgCACcCGUCc-- -3' miRNA: 3'- -UCGCGa--GCAUGGGCGa--GUG-GUAGacu -5' |
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| 151 | 3' | -56.4 | AC_000006.1 | + | 25775 | 0.7 | 0.317428 |
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Target: 5'- cGGCGCUUcccgGUGCCCGCUcCGCCcacaAUCgGGu -3' miRNA: 3'- -UCGCGAG----CAUGGGCGA-GUGG----UAGaCU- -5' |
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| 151 | 3' | -56.4 | AC_000006.1 | + | 9744 | 0.73 | 0.202459 |
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Target: 5'- uGGCGCUCGUGCagCCGCUCAggggCGUCgGGg -3' miRNA: 3'- -UCGCGAGCAUG--GGCGAGUg---GUAGaCU- -5' |
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| 151 | 3' | -56.4 | AC_000006.1 | + | 32826 | 1.09 | 0.00041 |
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Target: 5'- cAGCGCUCGUACCCGCUCACCAUCUGAg -3' miRNA: 3'- -UCGCGAGCAUGGGCGAGUGGUAGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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