miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1512 5' -56.4 NC_001335.1 + 44207 0.66 0.627888
Target:  5'- aGGGUGGCUGUUCGCgcagaggugUGAGGcGUGGugGa -3'
miRNA:   3'- -CUCGCUGGUAGGCG---------ACUCC-UACCugU- -5'
1512 5' -56.4 NC_001335.1 + 47878 0.67 0.572924
Target:  5'- cGAGgGACCGaaagaCGCUGuGGGAcGGGCAu -3'
miRNA:   3'- -CUCgCUGGUag---GCGAC-UCCUaCCUGU- -5'
1512 5' -56.4 NC_001335.1 + 12035 0.7 0.391735
Target:  5'- cGGGCGACCGcucuuguccuucgcgCCGUUGAGGAUcGGCu -3'
miRNA:   3'- -CUCGCUGGUa--------------GGCGACUCCUAcCUGu -5'
1512 5' -56.4 NC_001335.1 + 7480 0.72 0.292304
Target:  5'- cGGCGACCGUCUcgguGCcGGGGAugUGGGCGu -3'
miRNA:   3'- cUCGCUGGUAGG----CGaCUCCU--ACCUGU- -5'
1512 5' -56.4 NC_001335.1 + 46276 0.75 0.182432
Target:  5'- -uGCGGCCGUCgCGCUGAccGUGGGCAu -3'
miRNA:   3'- cuCGCUGGUAG-GCGACUccUACCUGU- -5'
1512 5' -56.4 NC_001335.1 + 12478 0.78 0.123934
Target:  5'- cGGCGAaaCCG-CCGUUGAGGGUGGACu -3'
miRNA:   3'- cUCGCU--GGUaGGCGACUCCUACCUGu -5'
1512 5' -56.4 NC_001335.1 + 41288 1.08 0.000757
Target:  5'- cGAGCGACCAUCCGCUGAGGAUGGACAc -3'
miRNA:   3'- -CUCGCUGGUAGGCGACUCCUACCUGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.