Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1514 | 3' | -53.4 | NC_001335.1 | + | 14590 | 0.66 | 0.769803 |
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Target: 5'- uGACGUAgucgaUGGGAuccucGGUGGUgaUCucGCGGAg -3' miRNA: 3'- gCUGCAU-----ACCCU-----UCACCG--AGu-CGUCU- -5' |
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| 1514 | 3' | -53.4 | NC_001335.1 | + | 45014 | 0.68 | 0.670206 |
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Target: 5'- aCGGCGUcgGGGAAcacaacugaauaUGGUUCcGCAGAc -3' miRNA: 3'- -GCUGCAuaCCCUUc-----------ACCGAGuCGUCU- -5' |
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| 1514 | 3' | -53.4 | NC_001335.1 | + | 38071 | 0.72 | 0.434669 |
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Target: 5'- cCGAgcUGUGUGGGcugccgacGGUggGGCUCAGCGGAg -3' miRNA: 3'- -GCU--GCAUACCCu-------UCA--CCGAGUCGUCU- -5' |
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| 1514 | 3' | -53.4 | NC_001335.1 | + | 9482 | 0.72 | 0.434669 |
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Target: 5'- gCGGCGcAUGGGAAGaGGC-CAuGCGGAu -3' miRNA: 3'- -GCUGCaUACCCUUCaCCGaGU-CGUCU- -5' |
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| 1514 | 3' | -53.4 | NC_001335.1 | + | 34421 | 0.72 | 0.424895 |
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Target: 5'- aGACGUucGGuGGAGUGGC-CAGCGGc -3' miRNA: 3'- gCUGCAuaCC-CUUCACCGaGUCGUCu -5' |
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| 1514 | 3' | -53.4 | NC_001335.1 | + | 41756 | 1.1 | 0.001236 |
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Target: 5'- cCGACGUAUGGGAAGUGGCUCAGCAGAa -3' miRNA: 3'- -GCUGCAUACCCUUCACCGAGUCGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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