Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1517 | 5' | -55.9 | NC_001335.1 | + | 31061 | 0.67 | 0.578005 |
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Target: 5'- uCUUGCgGUUcGgcCGGCUCGGGCCGGu -3' miRNA: 3'- -GAGUGgCAA-CauGCCGGGCCUGGUUu -5' |
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| 1517 | 5' | -55.9 | NC_001335.1 | + | 40959 | 0.68 | 0.545391 |
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Target: 5'- gUCGCCGUcguUGUGCGGgCCGGugaCGu- -3' miRNA: 3'- gAGUGGCA---ACAUGCCgGGCCug-GUuu -5' |
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| 1517 | 5' | -55.9 | NC_001335.1 | + | 41926 | 0.69 | 0.451929 |
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Target: 5'- aUCAgCCGUU-UGCGGCCUGG-CCGAu -3' miRNA: 3'- gAGU-GGCAAcAUGCCGGGCCuGGUUu -5' |
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| 1517 | 5' | -55.9 | NC_001335.1 | + | 7155 | 0.69 | 0.442075 |
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Target: 5'- --gGCCGa-GUACGGCCUGGACguGAa -3' miRNA: 3'- gagUGGCaaCAUGCCGGGCCUGguUU- -5' |
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| 1517 | 5' | -55.9 | NC_001335.1 | + | 7075 | 0.7 | 0.432344 |
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Target: 5'- aUCACCGUc--GCGGCCgucagccaGGACCAGAc -3' miRNA: 3'- gAGUGGCAacaUGCCGGg-------CCUGGUUU- -5' |
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| 1517 | 5' | -55.9 | NC_001335.1 | + | 32621 | 0.71 | 0.367912 |
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Target: 5'- -cCGCCGUUGgcCGGCgCGGAuCCAc- -3' miRNA: 3'- gaGUGGCAACauGCCGgGCCU-GGUuu -5' |
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| 1517 | 5' | -55.9 | NC_001335.1 | + | 43487 | 1.06 | 0.001224 |
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Target: 5'- gCUCACCGUUGUACGGCCCGGACCAAAa -3' miRNA: 3'- -GAGUGGCAACAUGCCGGGCCUGGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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