Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1518 | 5' | -54.9 | NC_001335.1 | + | 36447 | 0.66 | 0.738445 |
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Target: 5'- gUGGCuuGAUGcCcugGCGGUG-GGUGUGu -3' miRNA: 3'- aGCCG--CUACuGaa-CGCCACuCCACAC- -5' |
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| 1518 | 5' | -54.9 | NC_001335.1 | + | 51979 | 0.66 | 0.738445 |
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Target: 5'- gUGGUGGUGG--UGUGGUGuGcGUGUGu -3' miRNA: 3'- aGCCGCUACUgaACGCCACuC-CACAC- -5' |
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| 1518 | 5' | -54.9 | NC_001335.1 | + | 9877 | 0.66 | 0.70631 |
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Target: 5'- cCGGCuGUGGCUucgGCGGUGccGUGg- -3' miRNA: 3'- aGCCGcUACUGAa--CGCCACucCACac -5' |
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| 1518 | 5' | -54.9 | NC_001335.1 | + | 40681 | 0.66 | 0.70631 |
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Target: 5'- cCGGCGAUGAg-UGCGGUGGcGaUGa- -3' miRNA: 3'- aGCCGCUACUgaACGCCACUcC-ACac -5' |
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| 1518 | 5' | -54.9 | NC_001335.1 | + | 26621 | 0.68 | 0.607131 |
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Target: 5'- gUCGGCGAUGuAgUUGCGGacgGucuuGGUGcUGa -3' miRNA: 3'- -AGCCGCUAC-UgAACGCCa--Cu---CCAC-AC- -5' |
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| 1518 | 5' | -54.9 | NC_001335.1 | + | 44161 | 1.1 | 0.000888 |
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Target: 5'- aUCGGCGAUGACUUGCGGUGAGGUGUGg -3' miRNA: 3'- -AGCCGCUACUGAACGCCACUCCACAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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